Mouse Monoclonal Antibody to PTEN

PTEN (phosphatase and tensin homolog) was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. This protein is a phosphatidylinositol-3 4 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3 4 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.

1B8

Antibody are purified by protein G affinity chromatography. Liquid in PBS containing 50% glycerol and 0. 03% sodium azide.

1/200 - 1/400.
ELISA: Propose dilution 1/10000. Not yet tested in other applications. Determining optimal working dilutions by titration test. 1. Nat Genet. 1998 Aug; 19(4):348-55. 2. Oncogene. 1999 May 20; 18(20):3181-5. 3. Eur J Immunol. 2002 Apr; 32(4):1196-204.