AP2 alpha

GWB-E0FC2D

Synthetic peptide: SHTDNNAKSSDKEEKHRK conjugated to KLH corresponding to amino acids 420-437 of Human AP2 alpha.

Nuclear extract of HeLa human epithelioid carcinoma cells (western blotting) and of fixed cultured HeLa cells (immunofluorescence).

Nuclear

15mM Sodium Azide; Constituents: 1% BSA 0. 01M PBS. pH 7. 4

Nuclear Activation Protein transcription factor 2 (AP2) is a developmentally regulated transcription factor that recognizes a specific sequence CCCCAGGC or related sequences present in several promoter and enhancer gene regions including the promoters of the AP2 genes themselves. There are three different but highly homologous AP2 isoforms: alpha beta and gamma (ERF-1) encoded by three different retinoic acid-responsive genes. Each form of AP2 possesses the DNA binding domain (basic helix-span-helix motif) characteristic of this family of transcription factors. The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation viral transformation and oncogenesis. AP2 seems to play in important role in human breast cancer.

Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis.

The WW-binding motif mediates interaction with WWOX (By similarity).

Defects in TFAP2A are the cause of branchiooculofacial syndrome (BOFS) [MIM:113620]; also known as branchial clefts with characteristic facies growth retardation imperforate nasolacrimal duct and premature aging or lip pseudocleft-hemangiomatous branchial cyst syndrome. BOFS is a rare autosomal dominant cleft palate craniofacial disorder with variable expressivity. The major features include cutaneous anomalies ocular anomalies characteristic facial appearance (malformed pinnae oral clefts) and less commonly renal and ectodermal (dental and hair) anomalies.