PKC delta phospho S664

IgG

Human

K562 cells treated with PMA a phorbol ester

Cytoplasmic

0. 05% Sodium Azide; Constituents: 50% Glycerol PBS 1mg/ml BSA (IgG protease free). pH 7. 3

Cytoplasmic Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol phosphatidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop and is implicated in prolactin signaling.

ATP + a protein = ADP + a phosphoprotein.

Interacts with PDK1 RAD9A CDCP1 and MUC1.

The C1 domain containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B) is the diacylglycerol sensor.

Phosphorylated on Thr-507 within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity (By similarity).

Contains 1 AGC-kinase C-terminal domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.