Rabbit anti Cul3 (a.a. 1-12)

GWB-6F7700

A synthetic peptide corresponding to amino acids 1-12 of Human Cul3 (N-terminus) coupled to KLH

Cullin 3 (Cul3) N-terminal (a. a. 1-12)

Polyclonal Antibodies for Cancer Research

Not applicableApplications Notes : Suitable for use in ELISA (1:2 000 ? 1:10 000) and Western blot (1:500 ? 1:1 000). The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa or NIH-3T3). An 88. 9 kDa band corresponding to human Cul3 is detected. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4 H2AFY and DAXX and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition.

Forms neddylation-dependent homodimers. Component of multiple BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes formed of CUL3 RBX1 and a variable BTB domain-containing protein acting as both adapter to cullin and substrate recognition subunit. The BCR complex may be active as a heterodimeric complex in which NEDD8 covalently attached to one CUL3 molecule binds to the C-terminus of a second CUL3 molecule. Interacts with RBX1 RNF7 CYCE and TIP120A/CAND1. Part of the BCR(SPOP) containing SPOP. Part of the probable BCR(KLHL9-KLHL13) complex with BTB domain proteins KLHL9 and KLHL13. Part of the BCR(KBTBD10) complex containing KBTBD10. Part of the BCR(ENC1) complex containing ENC1. Part of a complex consisting of BMI1/PCGF4 CUL3 and SPOP. Part of a complex consisting of H2AFY CUL3 and SPOP. Interacts with KLHL9 KLHL13 GAN ZBTB16 KLHL21 KLHL3 KLHL15 KLHL20 C16orf44 GMCL1L BTBD1. Part of a complex that contains CUL3 RBX1 and GAN.

Widely expressed.

Belongs to the cullin family.