BMI-1


ArtNr	18-783-75571
Hersteller	GENWAY
Menge	0.1 mg
Kategorie	Oncology Antibodies Western Blot Primary Antibodies By Organ Lung Cancer
Typ	Antibody
Applikationen	WB
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-DDB17B
Similar products	18-783-75571
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Genway ID:	GWB-DDB17B
Specificity:	BMI-1NCBI
Gene ID:	648
Specificity:	BMI-1
Immunogen:	15 amino acid peptide sequence near the centre of human BMI-1.
Specificity Note:	This product recognises 37kDa polycomb complex protein BMI-1 involved in segment specification cell growth and maintenance transcriptional regulation and chromatin modification. BMI-1 is a proto-oncogene which cooperates with the myc oncogene to produce B-lymphomas. It is required in the regulation of normal adult and leukaemic stem cells making BMI-1 a potential target for stem cell proliferation and anti-cancer therapies. BMI-1 represses transcription at the ink4 locus which contains tumour suppressors p16 and p19Arf. BMI-1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex which ubiquitinates histone-2A at lys119 resulting in a heritable change in the ability of chromatin to express genes. BMI-1 is also required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2 within the PRC1 complex.
Antiserum Preparation:	Antisera to human BMI-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution:	Phosphate buffered saline
Preservative Stabilisers:		0. 02%Sodium AzideSuggested
Dilution:	Suggested
Dilution:	Western Blotting - 0. 5 - 1. 0ug/ml
Function:	Component of the Polycomb group (PcG) multiprotein PRC1 complex a complex required to maintain the transcriptionally repressive state of many genes including Hox genes throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A \' Lys-119\' rendering chromatin heritably changed in its expressibility. In the PRC1 complex it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Subunit:	Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110 BMI1/PCGF4 CBX2/M33 CBX4/PC2 CBX8/PC3 PHC1 PHC2 PHC3 SCMH1 RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1-like at least composed of PHC2 BMI1 RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1 CUL3 and SPOP.
Subcellular Location:	Nucleus.
Ptm:	May be polyubiquitinated; which does not lead to proteasomal degradation.
Disease:	Cooperates with the MYC oncogene to produce B-lymphomas.
Similarity:	Contains 1 RING-type zinc finger. 1. Raaphorst. F. et al. (2003) Self-renewal of hematopoietic and leukemic stem cells: a central role for the Polycomb-group gene Bmi-1. 2. Alkema. M. et al. (1993) Characterization and chromosomal localization of the human proto-oncogene BMI-1. 3. Lessard. J. et al. (2003) BMI-1 determines the proliferative capacity of normal and leukaemic stem cells.

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