PKR (N-term)

YE350

WB: 1:1 000-5 000IHC: 1:250-500ICC: 1:250-500IP: 1:100. Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation PKR is autophosphorylated at multiple sites and phosphorylates the a-subunit of the eukaryotic initiation factor-2 eIF2 (eIF2a) inhibiting protein synthesis (1 2). PKR controls the activation of several transcription factors (1) and mediates the effects of interferon via its capacity to inhibit protein synthesis (2). It has been shown that the autophosphorylation sites Thr-446 and Thr-451 in the activation loop of Human PKR are critical for its kinase activity (3). Does not react with Mouse or Rat. Not applicable in Flow Cytometry. 1. Willis KL Patel S Xiang Y et al The effect of the vaccinia K1 protein on the PKR-eIF2& Icirc; & plusmn; pathway in RK13 and HeLa cells Virology 394 (2009) 73-81[Application: WB]1. Williams B. R. PKR; a sentinel kinase for cellular stress. Oncogene 18: 61122. Cuddihy A. R. et al. The double-stranded RNA activated protein kinase PKR physically associates with the tumor suppressor p53 protein and phosphorylates human p53 on serine 392 in vitro. Oncogene 18:2690-2702 (1999). 3. Zhang F. et al. Binding of double-stranded RNA to protein kinase PKR is required for dimerization and promotes critical autophosphorylation events in the activation loop. J. Biol. Chem. 276: 249464. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487