IPP-1

GWB-A679E5

A synthetic peptide corresponding to residues in the N-terminus of human IPP-1 was used as an immunogen.

WB: 1:100 000IHC: 1:100-250FC: 1:80IP: 1:50. The inhibitor of protein phosphatase 1 (IPP-1 I-1) is an endogenous inhibitor of protein phophastase 1 (PP-1) a signal transducer in many cellular pathways. IPP-1 is a member of heat-stable PP1 inhibitory subunits which includes DARPP-32 (a homologue of IPP-1) and IPP-2. First discovered in rabbit skeletal muscle IPP-1 is expressed in various mammalian tissues. IPP-1 inhibition of PP-1 is controlled through phosphorylation at Thr35 by cAMP-dependent protein kinase and a tetra-peptide consensus motif in various PP-1 binding proteins (1). IPP-1 is a hormonal control in muscle contraction pituitary cell growth synaptic plasticity by neurotransmitters and glycogen metabolism (2). In vivo IPP-1 is phosphorylated at Ser-67 by NCLK which also initiates inhibition of PP-1 by IPP-1 (3). Triggered by increase in calcium level IPP-1 is inactivated by PP2B through de-phosphorylation (4). 1. Egloff MD et al. EMBO J 16:1876-1887 1997. 2. Oliver CJ Shenolikar S Front Biosci 3:D961-972 1998. 3. Huang K Paudel HK PNAS 97:5824-5829 2000. 4. Castellani GC Learn Mem 12:423-432 2005. 5. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487