Bcr Phospho (pY177)

GWB-38FFF8

A synthetic peptide corresponding to residues surrounding tyrosine 177 of human Bcr was used as immunogen.

WB: 1:1 000. The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML) (1-2). This 160 kDa protein contains a serine/threonine kinase domain an SH2 binding domain a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42 (3). Additionally Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling (4). 1. Clark S. S. McLaughlin J. Timmons M. Pendergast A. M. Ben-Neriah Y. Dow L. W. Crist W. Rovera G. Smith S. D. and Witte O. N. (1988). Science 239 775-7772. Konopka J. B. Watanabe S. M. and Witte O. N. (1984). Cell 37 1035-10422. Konopka J. B. Watanabe S. M. and Witte O. N. (1984). Cell 37 1035-10423. Boguski M. S. and McCormick F. (1993). Nature 366 643-654. 4. Braselmann S. and McCormick F. (1995). EMBO J. 14 4839-48485. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487