Plk1 [Mixed clones]

GWB-CD4771

IgGLight chain: kappa

Human

HeLa A431 and NIH 3T3 cells

Nuclear

0. 05% Sodium Azide; Constituents: 40% Glycerol PBS. pH 7. 4

Nuclear Polo-like kinases are a family of serine/threonine protein kinases named after the prototypic founding member of the family the polo gene product of Drosophila melanogaster. The polo kinase was originally identified in mutants that display abnormal mitotic spindle organization. Subsequently potential homologues of Drosophila polo have been identified in yeasts (Cdc5p in Saccharomyces cerevisiae; plo1+ in Schizosaccharmoyces pombe) and in mammals (polo-like kinase 1; Plk1). Genetic and biochemical studies suggest that polo Cdc5p and plo1+ may be required for mitotic spindle organization and possibly for cytokinesis. Likewise the patterns of expression activity and subcellular localization of Plk1 suggest that Plk12 may function during mitosis in spindle assembly and function. Recent studies have demonstrated that mammalian Plks regulate the function of the Golgi complex a cellular organelle closely associated with the centrosome and also have microtubule organization activity. Furthermore deregulated expression of human PLK1 and PLK3 is strongly correlated with the development of many types of malignancies and ectopic expression of kinase-active Plk3 or Plk1 dominant negative protein leads to rapid cell death.

ATP + a protein = ADP + a phosphoprotein.

Interacts with CEP170 and EVI5. Interacts and phosphorylates ERCC6L.

Placenta and colon. Developmental Stage: Accumulates to a maximum during the G2 and M phases declines to a nearly undetectable level following mitosis and throughout G1 phase and then begins to accumulate again during S phase.

Catalytic activity is enhanced by phosphorylation of Thr-210 and/or Ser-137.

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.

Contains 1 protein kinase domain.