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Bcl2 [SPM117] (ready-to-use)

Bcl2 [SPM117] (ready-to-use)

ArtNr	20-272-191153
Hersteller	GENWAY
Menge	7 ml
Kategorie	Oncology Antibodies Immunohistochemistry Primary Antibodies By Organ Colorectal Cancer
Typ	Antibody
Applikationen	IHC
Clon	SPM117
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-4FE782
Similar products	20-272-191153
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Genway ID:	GWB-4FE782
Clone:	SPM117
Isotype:	IgG1
Immunogen:	Synthetic peptide: GAAPAPGIFS SQPG corresponding to amino acids 41-54 of Human Bcl2.
Antigen Species:	Human
Positive Control:	Tonsil
Target:	Bcl2
Localization:	Endoplasmic reticulum outer mitochondrial membrane and nuclear membrane. Storage
Buffer:	PBS pH 7. 4 containing 1%BSA and 0. 1% sodium azide
Application Note:	IHC-P: Ready to use. Perform enzymatic antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer pH 6. 0 for 10 min followed by cooling at RT for 20 min. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular
Localization:	Endoplasmic reticulum outer mitochondrial membrane and nuclear membrane. Bcl2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of Bcl2 such as in the case of translocation of Bcl2 to Ig heavy chain locus is thought to be the cause of follicular lymphoma. Two transcript variants produced by alternate splicing differ in their C-terminal ends.
Function:	Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Subunit:	Forms homodimers and heterodimers with BAX BAD BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1 RAF-1 TP53BP2 BBC3 BCL2L1 MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.
Subcellular Location:	Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
Tissue Specificity:	Expressed in a variety of tissues.
Domain:	The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.
Ptm:	Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).
Ptm:	Proteolytically cleaved by caspases during apoptosis. The cleaved protein lacking the BH4 motif has pro-apoptotic activity causes the release of cytochrome c into the cytosol promoting further caspase activity.
Disease:	A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14; 18)(q32; q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Similarity:	Belongs to the Bcl-2 family.

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