DNA polymerase beta [18S]

18S

Full length protein (Rat).

Shows no effect on the polymerase activity of beta-pol.

DNA polymerase beta

10mM PBS pH7. 4 0. 2%BSA 0. 09% sodium azide

use at a concentration of 1 - 2µ g / ml for 2hrs at RT. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. By Western blot this antibody detects a band of 39 kDa which corresponds to the predicted molecular weight of DNA Polymerase beta. DNA polymerase beta comprises an amino-terminal 8 kDa domain and a carboxy terminal 31 kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyl transferase activity. Mammalian DNA polymerase beta a DNA repair polymerase is constitutively expressed in cultured cells but treatment of cells with the DNA-alkylating agents such as N-methyl-N\' -nitro-N-nitrosoguanidine (MNNG) or methyl methanesulphonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Cofactor: Binds 2 magnesium ions per subunit (By similarity).

Nucleus.

Belongs to the DNA polymerase type-X family.