Comparison

DNA polymerase beta [18S]

Item no. 20-272-192491
Manufacturer GENWAY
Amount 0.5 ml
Category
Type Antibody
Applications WB, IP
Clone 18S
Specific against other
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias GWB-651A32
Similar products 20-272-192491
Available
Genway ID:
GWB-651A32
Clone:
18S
Isotype:
IgG1
Immunogen:
Full length protein (Rat).
Antigen Species:
Rat
Specificity:
Shows no effect on the polymerase activity of beta-pol.
Positive Control:
HeLa cells
Target:
DNA polymerase beta
Concentration:
0. 2 mg/ml Storage
Buffer:
10mM PBS pH7. 4 0. 2%BSA 0. 09% sodium azide
Application Note:
Immunoprecipitation (denatured): use at a concentration of 2µ g / mg.
Western Blotting:
use at a concentration of 1 - 2µ g / ml for 2hrs at RT. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. By Western blot this antibody detects a band of 39 kDa which corresponds to the predicted molecular weight of DNA Polymerase beta. DNA polymerase beta comprises an amino-terminal 8 kDa domain and a carboxy terminal 31 kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyl transferase activity. Mammalian DNA polymerase beta a DNA repair polymerase is constitutively expressed in cultured cells but treatment of cells with the DNA-alkylating agents such as N-methyl-N\' -nitro-N-nitrosoguanidine (MNNG) or methyl methanesulphonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
Function:
Repair polymerase. Conducts \" gap-filling\" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5\' -deoxyribose-5-phosphate lyase (dRP lyase) activity.
Catalytic Activity:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Cofactor: Binds 2 magnesium ions per subunit (By similarity).
Subunit:
Monomer.
Subcellular Location:
Nucleus.
Domain:
Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity (By similarity).
Similarity:
Belongs to the DNA polymerase type-X family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.5 ml
Available: In stock
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