CNPase [11-5B]

11-5B

Full length native protein (purified) (Human).

Reacts specifically with CNPase. In an immunoblotting assay the antibody localizes both CNP1 (46kD) and CNP2 (48kD) bands. Immunohistochemical staining of paraffin cryostat or vibratome sections of rat brain reveals selective staining of oligodendrocytes in the grey and white matter. Nerve cells and axons are not stained and astroglial cells do not appear to be labelled.

CNPase Storage

Use at an assay dependant dilution. Dot: Use at an assay dependant dilution.
ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/500 for immunoblotting of fresh bovine whole brain extractNot tested in other applications. Optimal dilutions/concentrations should be determined by the end user. CNPase (2\' 3\' -Cyclic Nucleotide 3\' -Phosphodiesterase) exists in two forms: 46kDa (CNP 1) and 48kDa (CNP 2). It is expressed at high levels by oligodendrocytes in the central nervous system and by Schwann cells in the peripheral nervous system and is virtual absent in other celltypes. CNPase activity is decreased in demyelinating diseases such as multiple sclerosis. CNPase gene is localized to chromosome 17q21 very near the BRCA 1 familial breast cancer gene.

Membrane. Melanosome. Note=Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.