VCAM-1. CD106

GWB-4C5C51

The antibody is extremely useful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. Furthermore the antibody can be used for flow cytometry. immuno assays and immuno precipitation. Use: For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1% paraformaldehyde tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml) IL-1 (100 U/ml) or LPS (1ug/ml) in tissue culture medium at 37C. As negative control it is advised to use a control murine IgG1 antibody. The antibody reacts with VCAM-1. the vascular cell adhesion molecule. which is a member of the immunoglobulin superfamily. C2 subset. The antigen is induced on human endothelial cells by TNF-alpha. IL-1. IFN-gamma or endotoxin. The ligands for VCAM-1 are integrin alpha4. beta1 (VLA-4) and integrin alpha4. beta7. These integrins are constitutively expressed by thymocytes. lymphocytes and monocytes. Species: Mouse IgG1