Cytokeratin 7

GWB-81CB8C

RCK105

Each vial contains 100 ?l 1 mg/ml purified antibody in PBS containing 0. 09% sodium azide. Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity. represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4. 9 ? 7. 8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium. but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections. but are also a useful tool in cytopathology and flow cytometric assays.

Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7.

Expressed in cultured epidermal bronchial and mesothelial cells but absent in colon ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

Arg-20 is dimethylated probably to asymmetric dimethylarginine. Mass Spectrometry: Mass=51203. 48; Method=MALDI; Range=2-469; Source=PubMed:11840567; .

Belongs to the intermediate filament family. 1. Ramaekers. F. Huysmans. A. Schaart. G. Moesker. O. and Vooijs. P. (1987). Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp Cell Res 170. 235- 49. 2. Smedts. F. Ramaekers. F. Robben. H. Pruszczynski. M. van Muijen. G. Lane. B. Leigh. I. and Vooijs. P. (1990). Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol 136. 657-68. 3. Schaafsma. H. E. Ramaekers. F. C. van Muijen. G. N. Lane. E. B. Leigh. I. M. Robben. H. Huijsmans. A. Ooms. E. C. and Ruiter. D. J. (1990). Distribution of cytokeratin polypeptides in human transitional cell carcinomas. with special emphasis on changing expression patterns during tumor progression. Am J Pathol 136. 329-43. 5. Bauwens. L. J. De Groot. J. C. Ramaekers. F. C. Veldman. J. E. and Huizing. E. H. (1992). Expression of intermediate filament proteins in the adult human vestibular labyrinth. Ann Otol Rhinol Laryngol 101. 479-86. 6. Smedts. F. Ramaekers. F. Troyanovsky. S. Pruszczynski. M. Link. M. Lane. B. Leigh. I. Schijf. C. and Vooijs. P. (1992). Keratin expression in cervical cancer. Am J Pathol 141. 497-511. 7. Ivanyi. D. Minke. J. M. Hageman. C. Groeneveld. E. and van Doornewaard. G. (1992). Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. Am J Vet Res 53. 304-14.