TUBULIN ALPHA


ArtNr	20-783-72553
Hersteller	GENWAY
Menge	0.5 mg
Kategorie	Oncology Antibodies Cell Biology Cytoskeleton Immunohistochemistry Immunoprecipitation Western Blot ELISA Immunoassay Primary Antibodies Mikrotubules By Organ Heart & Blood Cancer
Typ	Antibody
Applikationen	WB, IP, IHC, ELISA, RIA
Clon	YL1/2
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-80936D
Similar products	20-783-72553
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Genway ID:	GWB-80936D
Specificity:	TUBULIN ALPHA
Specificity:	TUBULIN ALPHA
Clone:	YL1/2
Immunogen:	Yeast tubulin
Fusion Partner:	Spleen cells from immunised LOU rats were fused with cells of the Y3. Ag. 1. 2. 3 rat myeloma cell line.
Specificity Note:	This product recognises the alpha subunit of tubulin specifically binding tyrosylated Tubulin (Tyr-Tubulin) (2). The epitope recognised by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances including E. coli rec A and oxidized actin (5). This product is routinely tested in ELISA on Tubulin.
Preparation:	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution:	Phosphate buffered saline pH7. 4
Preservative Stabilisers:		0. 09%Sodium AzideSuggested
Dilution:	Suggested
Dilution:	ELISA - 1/100 - 1/1000 1. Kilmartin. J. V. et al. (1982) Rat monoclonal anti tubulin antibodies derived by using a new non secreting rat cell line. 2. Wehland. J. et al. (1983) A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. I. Biochemical characterization. effects on microtubule polymerization in vitro. and microtubule polymerization and organization in vivo 3. Wehland. J. et al. (1984) Amino acid sequence requirements in the epitope recognized by the alpha tubulin specific rat monoclonal antibody YL1/2. 4. Burns. R. (1987) Cytoskeleton. Tubulin\' s terminal tyrosine 5. Skinner. R. H. et al. (1991) Use of the Glu-Glu-Phe C-terminal epitope for rapid purification of the catalytic domain of normal and mutant ras GTPase-activating proteins.

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Menge: 0.5 mg

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