CD44


ArtNr	20-783-72670
Hersteller	GENWAY
Menge	100 TESTS
Kategorie	Oncology Antibodies Flow Cytometry Primary Antibodies By Organ Gastric Cancer
Typ	Antibody
Applikationen	FC
Clon	F10-44-2
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-53228B
Similar products	20-783-72670
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Genway ID:	GWB-53228B
Specificity:	CD44
Specificity:	CD44
Clone:	F10-44-2
Immunogen:	Human T lymphocytes
Fusion Partner:	Spleen cells of immunised BALB/c mice were fused with cells from the mouse NS1 myeloma line.
Specificity Note:	This product recognises the human CD44 cell surface antigen a 100kD glycoprotein widely expressed on human leucocytes white matter of the brain and by some epithelial cells of the intestine and of the breast. CD44 expression may be up-regulated upon some carcinomas and it has been speculated that this may be related to metastatic potential. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Preparation:	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:	Phosphate buffered saline pH7. 2
Preservative Stabilisers:		0. 09%Sodium Azide1%Bovine Serum Albumin Reconstitution: Reconstitute with 1 ml distilled waterSuggested
Flow Cytometry:	Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested
Dilution:	Flow Cytometry - Neat
Function:	Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA and possibly also through its affinity for other ligands such as osteopontin collagens and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration tumor growth and progression. Also involved in lymphocyte activation recirculation and homing and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Subunit:	Interacts with HA as well as other glycosaminoglycans collagen laminin and fibronectin via its N-terminal segment. Interacts with ANK the ERM proteins (VIL2 RDX and MSN) and NF2 via its C-terminal segment.
Subcellular Location:	Membrane; Single-pass type I membrane protein.
Tissue Specificity:	An epithelial isoform (CD44E) is expressed by cells of epithelium and highly expressed by carcinomas. An hematopoietic isoform (CD44H) is expressed by cells of mesodermal origin. Expression is repressed in neuroblastoma cells.
Ptm:	Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
Ptm:	N-glycosylated.
Ptm:	O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Ptm:	Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site) and the phosphorylation of Ser-672.
Polymorphism:	CD44 is responsible for the Indian blood group system. The molecular basis of the In(A)=In1/In(B)=In2 blood group antigens is a single variation in position 46; In(B) the most frequent allele has Arg-46.
Similarity:	Contains 1 Link domain. 1. Dalchau. R. et al. (1980) Monoclonal antibody to a human brain granulocyte T lymphocyte antigen probably homologous to the W3/13 antigen of the rat. 2. Daar. A. S. and Fabre. J. W. (1981) Demonstration with monoclonal antibodies of an unusual mononuclear cell infiltrate and loss of normal epithelial membrane antigens in human breast carcinomas 3. Cattoretti. G. et al. (1993) Antigen unmasking on formalin-fixed. paraffin-embedded tissue sections 4. Yoshino. N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts. cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. 5. Roscic - Mrkic. B. et al. (2003) RANTES (CCL5) uses the proteoglycan CD44 as an auxiliary receptor to mediate cellular activation signals and HIV - 1 enhancement.

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