Human Growth Hormone Hormone ELISA Kit

GWB-FE912C

Growth FactorsSample Type: Serum and plasmaSensitivity: 0 2 IU/mLDescription: Immunoenzymetric assay for the in vitro quantitative measurement of human Growth Hormone (hGH) in serum and plasma.
Intended Use: Immunoenzymetric assay for the in vitro quantitative measurement of human Growth Hormone (hGH) in serum and plasma. Background: A. Biological activitieshGH is a polypeptide hormone (molecular weight 21 500 Da) produced by the acidophil cells of the anterior pituitary under the control of two main substances from the median eminence : Growth-hormone Releasing Factor (GRF) and an inhibitory agent somatostatin. Dopaminergic adrenergic and serotoninergic neuroendocrine pathways also play an important role in the control of hGH secretion. Excitatory stimuli of hGH secretion include hypoglycemia exercise fasting meals with a high protein content deep sleep stress glucagon L Dopa amino acids etc. Inhibitory stimuli include glucose cortisol hGH and free fatty acids. Because of its short plasma half life (& plusmn; 25 minutes) and of the frequent excitatory or inhibitory stimuli hGH displays frequent and large variations of concentration in serum. One of the main physiological functions of hGH is to act on the liver and other tissues to produce Somatomedins which in turn induce growth by direct action on target tissues. In contrast to hGH the concentration of somatomedin in serum is kept stable by virtue of being largely bound to circulating plasma proteins. Principles of the method: \' s HGH-EASIA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on a microtiterplate. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 - hGH - MAb 2 - HRP the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzyme-labelled antibody is measured through a chromogenic reaction. The revelation solution (TMB - H2O2) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance which is proportional to the hGH concentration. A calibration curve is plotted and hGH concentration in samples is determined by interpolation from the calibration curve. The use of the EASIA reader (linearity up to 3 OD units) and a sophisticated data reduction method (polychromatic data reduction) result in a high sensitivity in the low range and in an extended calibration range. Storage and Stability: ? Before opening or reconstitution all kits components are stable until the expiry date indicated on the vial label if kept at 2 to 8C. ? Unused strips must be stored at 2-8C in a sealed bag containing a desiccant until expiration date. ? After reconstitution calibrators and controls are stable for 1 week at 2 to 8C. For longer storage periods aliquots should be made and kept at -20C. Avoid successive freeze thaw cycles. ? The concentrated Wash Solution is stable at room temperature until expiration date. ? Freshly prepared Working Wash solution should be used on the same day. ? After its first use the conjugate is stable until expiry date if kept in the original well-closed vial at 2 to 8C. ? The freshly prepared revelation solution is stable before use for maximum 15 minutes at room temperature and must be discarded afterwards. ? Alterations in physical appearance of kit reagents may indicate instability or deterioration.

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

Secreted.

Defects in GH1 are the cause of Kowarski syndrome [MIM:262650]; also known as pituitary dwarfism VI.

Defects in GH1 may be a cause of short stature [MIM:604271]. Short stature is defined by a subnormal rate of growth. Pharmaceutical: Available under the names Nutropin or Protropin (Genentech) Norditropin (Novo Nordisk) Genotropin (Pharmacia Upjohn) Humatrope (Eli Lilly) and Saizen or Serostim (Serono). Used for the treatment of growth hormone deficiency and for Turner\' s syndrome.

Belongs to the somatotropin/prolactin family.