NFkappaB-p100/p52 (Ser869) Antibody Blocking Peptide

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation immunity differentiation cell growth tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65 RELB NFKB1/p105 NFKB1/p50 REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5\' -GGRNNYYCC-3\' located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex which stimulates the HIV enhancer in synergy with p65.

Nucleus. Cytoplasm. Note=Nuclear but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).

The glycine-rich region (GRR) appears to be a critical element in the generation of p52.

Subsequent to MAP3K14-dependent serine phosphorylation p100 polyubiquitination occurs then triggering its proteasome-dependent processing.

A chromosomal aberration involving NFKB2 is found in a case of B-cell non Hodgkin lymphoma (B-NHL). Translocation t(10; 14)(q24; q32) with IGHA1. The resulting oncogene is also called Lyt-10C alpha variant.

In B-cell leukemia (B-CLL) cell line LB40 and EB308 can be found after heterogeneous chromosomal aberrations such as internal deletions.

Contains 1 death domain.