GroE Mix

GFK

GroE Mix is a supplement for the PUREfrex® series reconstituted coupled transcription/translation systems, designed to promote proper folding and solubility of proteins that require molecular chaperones for proper protein folding. GroE Mix comprises an optimized ratio of highly purified E.coli-derived GroEL (Hsp60) and GroES.
Chaperone Supplement
DnaK Mix comprises highly purified and ratio-optimized E. coli DnaK, DnaJ and GrpE. DnaK, known as Hsp70, has ATPase activity and is stimulated by co-chaperones DnaJ and GrpE. DnaJ facilitates DnaK ATPase activity and binds to hydrophobic regions of synthesized proteins. GrpE stimulates ADP/ATP exchange of DnaK. DnaK Mix works well with PUREfrex® series and DsbC Set.
Chaperone Supplement
GroE Mix comprises highly purified and ratio-optimized E. coli GroEL (known as Hsp60) and GroES (works in conjunction with GroEL).
Reagent
Qty
Description
Storage
GroE Mix
12.5 ul
20µ M GroEL, 14-mer form, and 40µ M GroES, 7-mer form, from E.coli in 30% glycerol buffer (No tags)
-80 C
Dilution Buffer
500 ul
30% glycerol buffer
-20 C
Reagent
Qty
Description
Storage
DnaK Mix
25 ul
100µ M DnaK, 20µ M DnaJ and 20µ M GrpE from E.coli in 30% glycerol buffer (No tags)
-80 C
Dilution Buffer
500 ul
30% glycerol buffer
-20 C