CTSE Antibody (Center)

Synthetic Peptide

CTSE is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen.

Polyclonal

1510

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-CTSE Antibody (Center) at 1：500-1：2000 dilution
Lane 1: Mouse spleen whole tissue lysate
Lane 2: MKN45 whole cell lysate
Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1：10000 dilution.

Predicted band size : 43 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1：500-1：2000

WB

All lanes : Anti-CTSE Antibody (Center) at 1:2000 dilution
Lane 1: MGC803 whole cell lysate
Lane 2: MKN45 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 43 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

IHC-P

CTSE Antibody (Center) (Cat. #AP6568c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

1:10~50

42794

HUMAN

NP_001901.1; NP_683865.1