HK1 (Hexokinase) Antibody (N-term)

Synthetic Peptide

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. The hexokinase gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of the hexokinase gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. HK1 encodes the ubiquitously expressed isoform. Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane.

Polyclonal

3098

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-HK1 (Hexokinase) Antibody (N-term) at 1:1000 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: Rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 110 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:1000

WB

Western blot analysis of HK1 (arrow) using HK1 Antibody (N-term) (Cat.#AP8141a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).

1:1000

WB

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using AP8141a performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

1:500

102486

HUMAN

NP_000179.2; NP_277031.1; NP_277032.1; NP_277033.1; NP_277035.2