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IFNB1 Antibody (N-term)

IFNB1 Antibody (N-term)

ArtNr	ABC-AP8539a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	IFN-beta, IFNB1, IFNB, IFB, Interferon beta, Fibroblast interferon
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Primary Accession	P01574
Antigen Type	Synthetic Peptide
Application	IHC-P, WB, FC
Bio Background	IFNB1 has antiviral, antibacterial and anticancer activities.
Bio References	Lienenklaus, S., et.al., J. Immunol. 183 (5), 3229-3236 (2009) Eto, T.et.al., Nat. Med. 5 (5), 577-581 (1999)
Clonality	Polyclonal
Clone/Animal Names	RB22040
Gene ID	3456
Gene Name	IFNB1
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of IFNB1 Antibody (N-term) (Cat. #AP8539a) in HepG2 cell line lysates (35ug/lane). IFNB1 (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Anti-IFNB1 Antibody (N-term) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	Formalin-fixed and paraffin-embedded human hepatocarcinoma with IFNB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Legend image 4	Overlay histogram showing HepG2 cells stained with AP8539a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP8539a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 4	FC
Dilution image 4	1:25
Other Accession	O77812
Predicted	Pr
Isotype	Rabbit IgG
Calculated MW	22294
Antigen Region	39-66
Antigen Source	HUMAN
Target/Specificity	This IFNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the N-terminal region of human IFNB1.
NCBI Accession	NP_002167.1

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ABC-AP8539a-datasheet.pdf

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Menge: 400 ul

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