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IFNB1 Antibody (N-term)

IFNB1 Antibody (N-term)

Item no.	ABC-AP8539a
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	IFN-beta, IFNB1, IFNB, IFB, Interferon beta, Fibroblast interferon
Available
Primary Accession	P01574
Antigen Type	Synthetic Peptide
Application	IHC-P, WB, FC
Bio Background	IFNB1 has antiviral, antibacterial and anticancer activities.
Bio References	Lienenklaus, S., et.al., J. Immunol. 183 (5), 3229-3236 (2009) Eto, T.et.al., Nat. Med. 5 (5), 577-581 (1999)
Clonality	Polyclonal
Clone/Animal Names	RB22040
Gene ID	3456
Gene Name	IFNB1
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of IFNB1 Antibody (N-term) (Cat. #AP8539a) in HepG2 cell line lysates (35ug/lane). IFNB1 (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Anti-IFNB1 Antibody (N-term) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	Formalin-fixed and paraffin-embedded human hepatocarcinoma with IFNB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Legend image 4	Overlay histogram showing HepG2 cells stained with AP8539a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP8539a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 4	FC
Dilution image 4	1:25
Other Accession	O77812
Predicted	Pr
Isotype	Rabbit IgG
Calculated MW	22294
Antigen Region	39-66
Antigen Source	HUMAN
Target/Specificity	This IFNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the N-terminal region of human IFNB1.
NCBI Accession	NP_002167.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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