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Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin Europäischer Partner

ArtNr ICH1044-1MG
Hersteller IchorBio
Menge 1 mg
Quantity options 100 mg 1 mg 25 mg 50 mg 5 mg
Kategorie
Typ Antibody
Applikationen WB, FC, IP, IHC
Clon 53-6.7
Specific against Mouse (Murine, Mus musculus)
Host Rat
Isotype IgG2a Kappa
Purity >95% by SDS-PAGE and HPLC
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias CD8 alpha chain, Lyt-2, Lyt2
Versandbedingung Gekühlt
Lieferbar
Specificity Anti-CD8a In Vivo Antibody - Low Endotoxin (53-6.7) recognizes Lyt 2, lymphocyte differentiation antigen. The antibody precipitates 44 kDa and 33 kDa proteins and reacts with non-polymorphic determinant.
Manufacturer - Type
Low Endotoxin, anti-mouse
Manufacturer - Applications
Immunoprecipitation, Western Blot, Flow Cytometry, CyTOF, IHC (Frozen)
Manufacturer - Category
Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype
Rat IgG2a Kappa
Manufacturer - Targets
CD8a
Shipping Temperature
Blue ice
Storage Conditions
anti-CD8a In Vivo Antibody - Low Endotoxin (53-6.7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation
Aggregation level ≤ 5%
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
Present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.
Application Notes
Immunoprecipitation: Rat Anti-Mouse CD8a (Clone 53-6.7) precipitates 44 kDa and 33 kDa proteins.
Each investigator should determine their own optimal working dilution for specific applications.
Background
CD8, a.k.a Lyt-2, Ly-2, or T8, consists of disulfide-linked A and β chains that form the A(CD8a)/β(CD8b) heterodimer and A/A homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 A/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR A/β T cells.
Concentration
1.0 - 5.0 mg/ml
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Manufacturer - Specificity
Anti-CD8a In Vivo Antibody - Low Endotoxin (53-6.7) recognizes Lyt 2, lymphocyte differentiation antigen. The antibody precipitates 44 kDa and 33 kDa proteins and reacts with non-polymorphic determinant.
Purification method
This monoclonal antibody was purified using Protein G
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen
Mouse thymus or spleen
Related Antibodies
Antibodies against the same target (Cat No.)
Isotype Control
Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)
Buffer
ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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