Anti-IFN Beta (HDB-4A7) In Vivo Antibody - Ultra-Low Endotoxin

Western Blot, Blocking, Functional Assays

Mouse IgG2a

Blue ice

Aggregation level ≤ 1%

Functional Assay: In Vitro Activity of HDB-4A7 - inhibits IFNb-induced Stat1 phosphorylation (level of inhibition is similar to that seen using anti-IFNAR1 mAb (MAR1-5A3). - blocks IFNb induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines. - neutralizes IFNb-induced antiviral activity in vitro following infection of L929 cells with VSV In Vivo Activity of HDB-4A7 - circulates with a half-life of ~ 14 days. - blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of IFNb-/- mice.
Each investigator should determine their own optimal working dilution for specific applications.

≤ 0.5 EU/mg as determined by the LAL method

This monoclonal antibody was purified using Protein A

Plasmid DNA encoding murine IFNb

Anti-IFN beta In Vivo Antibody - Low Endotoxin [HDB-4A7] (ICH1110)

ICH3001-100ml; ICH3002-100ml; ICH3003-100ml