LinKine Cy3 Labeling Kit (Suitable for samples with molecular weight of 60KD to 100KD)

LinKine Cy3 Labeling Kit (Suitable for samples with molecular weight of 60KD to 100KD) is designed for preparing Cy3 conjugates directly from proteins, peptides, and other ligands that contain a free amino group.

- Activated Cy3 solution
- Cy3 labeling solution
- Purification column (0.5 mL, 30KD)

- The initial concentration of the sample to be labelled should not less than 2 mg/ml.
- Please note that components that containing amino are not allowed in the buffer, which will affect the coupling effects.
- A small amount of BSA will not affect the results, and PBS is recommended to use as the required buffer.

Gel pack with blue ice.

Cyanines dyes are usually synthesized from 2, 3, 5 or 7 methine structures with reactive groups on either one or both of the nitrogen side chains so that they can be chemically linked to either nucleic acids or protein molecules. Labeling is done for visualization and quantification purposes. Biological applications include comparative genomic hybridization and gene chips, which are used in transcriptomics, and various studies in proteomics such as RNA localization, molecular interaction studies by fluorescence energy transfer (FRET) and fluorescent immunoassays. Cy3 is a fluorescent dye, often used in fluorescence microscopy applications and in immunohistochemistry. Cy3 fluoresces greenish yellow (ca.548 nm excitation, ca.565 nm emission) Due to inherently high extinction coefficient, this dye is also easily detected by naked eye on gels, and in solution. Labeled antibodies can be used in many techniques such as flow cytometry.

Cy3