PurKine Antibody Purification Protein A/G Kit

- High capacity—More than 7mg human lgG per mL of resin.
- High performance—Recombinant Protein A/G Beads combines the IgG binding domains of both Protein A and Protein G. Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a more general and convenient tool for investigating and purifying immunoglobulins.
- Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
- Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
- Robust—highly crosslinked beads tolerate linear flow rates up to 300cm/hour.
- Easy to use— Pre-formulated buffers available for kit formats.

PurKine Protein A/G Purification system is effective for affinity purification of IgG from serum, ascites fluid, cell culture supernantant and other antibody samples. Recombinant Protein A/G is chemically modified with a proprietary method to minimize nonspecific binding of proteins. Also, Protein A/G binding to immunoglobulins is not as pH-dependant as Protein A. Protein A/G binds to all human IgG subclasses and binds well to all mouse IgG subclasses but does not bind mouse IgA, IgM or serum albumin. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine Protein A/G Purification Resin is also available as prepacked spin column and kit formats.