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Anti-Respiratory Syncytial Virus (Clone: RSV-12I1) - Biotin - 250 µg

ArtNr LEIN-R223-250ug
Hersteller Leinco Technologies
Menge 250 ug
Quantity options 100 ug 250 ug
Kategorie
Typ Antibody Monoclonal
Applikationen WB, ELISA, FA
Clon RSV-12I1
Specific against other
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Applications
ELISA, FA, N, WB
Manufacturer - Category
Primary Monoclonal Antibodies>Infectious Diseases|Monoclonal Antibodies|Recombinant Antibodies
Manufacturer - Targets
RSV
Shipping Temperature
Standard Overnight on Blue Ice.
Storage Conditions
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description
Respiratory syncytial virus (RSV) is a common respiratory virus that infects the majority of children by two years old1, 2. While usually mild, RSV can be serious in infants and older adults and is the leading cause of bronchiolitis and pneumonia in children less than one year of age in the United States1. Antibodies have been described that bind and neutralize RSV fusion (F) protein2-4. RSV F protein is a type I integral membrane protein that is synthesized as a 574 amino acid inactive precursor, assembled into a trimer, post-translationally modified, then cleaved to produce F1, F2, and intervening peptide pep273. Functional F protein has both pre- and post-fusion conformations. RSV F protein is highly conserved among RSV isolates from both A and B subgroups3 and is the primary target for antiviral drug development3 with several antigenic regions capable of introducing neutralizing antibodies2. RSV-12I1 was generated from human donors using human hybridoma technology4. Transformed B cells were screened by ELISA against recombinant post-fusion RSV A2 F protein and isolated by single-cell flow cytometric sorting. RSV-12I1 failed to show neutralization activity against RSV strain A2 in a plaque reduction neutralization assay. RSV-12I1 did bind to both pre- and post-fusion F protein in an ELISA binding assay, favoring the post-fusion conformation. Competition-binding studies showed that RSV-12I1 targets antigenic site II, which is the target of palivizumab, an antiviral monoclonal antibody licensed as a prophylactic treatment. RSV-12I1 readily competed with RSV-14N4 on post-fusion F, but the competition was less pronounced on pre-fusion F. RSV-12I1 also competed with palivizumab on post-fusion F in a palivizumab competition assay. Saturation alanine scanning mutagenesis identified residues Leu467 and Lys470 as critical for RSV-12I1 binding. Binding was not detected to scaffolded epitopes containing site II. The RSV-12I1 isotype is IgG1.
Manufacturer - Research Area
Infectious Disease, Respiratory Syncytial, Seasonal and Respiratory Infections, Viral, IVD Raw Material
Concentration
0.5 mg/ml
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Antigen Distribution
F protein is a surface glycoprotein
Keywords
Anti-Respiratory Syncytial Virus, RSV-12I1, RSV, Orthopneumovirus

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 250 ug
Lieferbar: In stock
lieferbar

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