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Human Arginase 2/ARG2 antibody

ArtNr NKM-ATGA0389-100ul
Hersteller NKMAX
Menge 100 ul
Quantity options 100 ul 50 ul
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, FC, ICC, ELISA
Clon AT7F8
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Konjugat/Tag Unconjugated
Citations Watts JA., et al. (2011) Pulm Pharmacol Ther. 24(4): 407-413.
Tadie JM., et al. (2008) Am J Physiol Lung Cell Mol Physiol. 294(3): 489-497.
NCBI NP_001163
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Arginase-2 type II,Kidney arginase,Non-hepatic arginase,Arginase-2,type II Arginase II mitochondrial,Kidney type arginase,Non hepatic arginase.
Lieferbar
Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, WB, ICC/IF, FACS
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
Arginase 2 catalyzes the same reaction as arginase 1, but differs in its tissue specificity and subcellular location. Specifically, arginase 2 localizes to the mitochondria. Arginase 2 is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase 1, is not expressed in liver.
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human ARG2 (23-354aa) purified from E. coli
Ab Usage
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-A affinity chromatography
Gene Category
Metabolism
Manufacturer - Search Terms
Arginase2, ARG2, Arginase-2 type II, Kidney arginase, Non-hepatic arginase, Arginase-2, type II Arginase II mitochondrial, Kidney type arginase, Non hepatic arginase., ATGA0389-10ug, ATGA0389-20ug, ATGA0389-50ug, ATGA0389-100ug, ATGA0389-250ug, ATGA0389-500ug, ATGA0389-1mg, ATGA0389-10, ATGA0389-20, ATGA0389-50, ATGA0389-100, ATGA0389-250, ATGA0389-500, ATGA0389-1
Manufacturer - Similar Names
Arginase2, ARG2, Arginase-2 type II, Kidney arginase, Non-hepatic arginase, Arginase-2, type II Arginase II mitochondrial, Kidney type arginase, Non hepatic arginase., ATGA0389-10ug, ATGA0389-20ug, ATGA0389-50ug, ATGA0389-100ug, ATGA0389-250ug, ATGA0389-500ug, ATGA0389-1mg, ATGA0389-10, ATGA0389-20, ATGA0389-50, ATGA0389-100, ATGA0389-250, ATGA0389-500, ATGA0389-1
IF Comment
ICC/IF analysis of ARG2 in SW480 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ARG2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
WB Comment
The SW480 Cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARG2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : SW480 cell lysate The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARG2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: ARG2 recombinant protein (ATGP0591)
Lane 2.: ARG1 recombinant protein (ATGP0564)
FACS Comment
Flow cytometry analysis of ARG2 in SW480 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ul
Lieferbar: In stock
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