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Recombinant human TNF-alpha protein

Recombinant human TNF-alpha protein

ArtNr	NKM-TNF0501-100ug
Hersteller	NKMAX
Menge	100 ug
Quantity options	100 ug 20 ug 250 ug 500 ug 50 ug
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis
Typ	Proteins Recombinant
Format	Liquid
Applikationen	SDS-PAGE
Specific against	Human (Homo sapiens)
Host	E.coli
Konjugat/Tag	Untagged
Purity	> 95% by SDS-PAGE
Sequence	MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Citations	Chicoine M.R., et al. (1997), J. Neurooncol. 35: 249-57 Ruddle N.H., et al. (1992), Curr. Opin. Immunol. 4: 327-32 Wingfield P., et al. (1987), FEBS Lett. 211:179-184 Kim JA, et al. A new lupane-type triterpene from the seeds of Panax ginseng with its inhibition of NF-κB. (Arch Pharm Res. 2012){https://pubmed.ncbi.nlm.nih.gov/22553057/} Nhiem NX, et al. Inhibition of nuclear transcription factor-κB and activation of peroxisome proliferator-activated receptors in HepG2 cells by cucurbitane-type triterpene glycosides from Momordica charantia. (J Med Food. 2012){https://pubmed.ncbi.nlm.nih.gov/22248180/} Song SB, et al. Inhibition of TNF-α-mediated NF-κB Transcriptional Activity in HepG2 Cells by Dammarane-type Saponins from Panax ginseng Leaves. (J Ginseng Res. 2012){https://pubmed.ncbi.nlm.nih.gov/23717114/} Liang C, et al. Oleanane-triterpenoids from Panax stipuleanatus inhibit NF-κB. (J Ginseng Res. 2013){https://pubmed.ncbi.nlm.nih.gov/23717159/} Cho K, et al. Inhibition of TNF-α-Mediated NF-κB Transcriptional Activity by Dammarane-Type Ginsenosides from Steamed Flower Buds of Panax ginseng in HepG2 and SK-Hep1 Cells. (Biomol Ther (Seoul). 2014){https://pubmed.ncbi.nlm.nih.gov/24596622/} Thao NP, et al. Inhibition of NF-κB transcriptional activation in HepG2 cells by diterpenoids from the soft coral Sinularia maxima. (Arch Pharm Res. 2014){https://pubmed.ncbi.nlm.nih.gov/23934574/} Thao NP, et al. NF-κB inhibitory activity of polyoxygenated steroids from the Vietnamese soft coral Sarcophyton pauciplicatum. (Bioorg Med Chem Lett. 2014){https://pubmed.ncbi.nlm.nih.gov/24852121/} Li W, et al. Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana. (Biomol Ther (Seoul). 2014){https://pubmed.ncbi.nlm.nih.gov/25143813/} He F, et al. Antitumor effects of dammarane-type saponins from steamed Notoginseng. (Pharmacogn Mag. 2014){https://pubmed.ncbi.nlm.nih.gov/25210319/} Yang SY, et al. NF-κB Activation and PPAR Transactivational Effects of a New Aliphatic Acid Amide from Pericarps of Zanthoxylum piperitum. (Bull Korean Chem Soc. 2014){https://doi.org/10.5012/bkcs.2014.35.8.2361} Sun YN, et al. NF-κB Inhibitory Activities of Phenolic and Lignan Components from the Stems of Acanthopanax divaricatus var. albeofructus. (Natural Product Sciences. 2014){https://koreascience.kr/article/JAKO201402852974800.page} Sun YN, et al. Nuclear Factor Kappa B Activation and Peroxisome Proliferator-activated Receptor Transactivational Effects of Chemical Components of the Roots of Polygonum multiflorum. (Pharmacogn Mag. 2016){https://pubmed.ncbi.nlm.nih.gov/27019559/} Sun YN, et al. Isolation and identification of chromone and pyrone constituents from Aloe and their anti-inflammatory activities. (J Funct Foods. 2016){https://doi.org/10.1016/j.jff.2015.12.026} Sun YN, et al. A new phenolic derivative with soluble epoxide hydrolase and nuclear factor-kappaB inhibitory activity from the aqueous extract of Acacia catechu. (Nat Prod Res. 2016){https://pubmed.ncbi.nlm.nih.gov/26647286/} Kwak MS, et al. Peroxiredoxin-mediated disulfide bond formation is required for nucleocytoplasmic translocation and secretion of HMGB1 in response to inflammatory stimuli. (Redox Biol. 2019){https://pubmed.ncbi.nlm.nih.gov/31026770/} Jang SH, et al. Sinapic acid alleviates inflammatory bowel disease (IBD) through localization of tight junction proteins by direct binding to TAK1 and improves intestinal microbiota. (Front Pharmacol. 2023){https://pubmed.ncbi.nlm.nih.gov/37649894/} Kim S, et al. Hybrid nutraceutical of 2-ketoglutaric acid in improving inflammatory bowel disease: Role of prebiotics and TAK1 inhibitor. (Biomed Pharmacother. 2024){https://pubmed.ncbi.nlm.nih.gov/38219386/}
NCBI	NP_000585.2
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Tumor necrosis factor,Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2,TNFSF2,TNF-a,TNFA,TNF
Similar products	TNF-alpha, TNF, TNF-a, TNFA, TNFSF2, DIF, member 2), TNF superfamily member 2, Tumor necrosis factor alpha, DIF (differentiation inducing factor ), CACHECTIN, tumor necrosis factor (TNF superfamily, TNF0501-10ug, TNF0501-20ug, TNF0501-50ug, TNF0501-100ug, TNF0501-250ug, TNF0501-500ug, TNF0501-1mg, TNF0501-10, TNF0501-20, TNF0501-50, TNF0501-100, TNF0501-250, TNF0501-500, TNF0501-1
Lieferbar
Manufacturer - Type	Recombinant Protein
Manufacturer - Applications	SDS-PAGE, Bioactivity
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Molecular Weight	17.5 kDa (158aa) confirmed by MALDI-TOF
Description	Tumor necrosis factor alpha (TNF-alpha), also called cachectin, consists of 157 amino acids. TNF-alpha is a 17. 5 kD factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-alpha is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Recombinant human TNF-alpha was expressed in E. coli and purified by using conventional chromatography techniques. Additional amino acid, methionine, was attached at N-terminus of the protein.
Concentration	1mg/ml (determined by Bradford assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4)
Antigen Species	Human
Gene Category	Hormones & Cytokines
Domain	77-233aa
Endotoxin	< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity Comment Only	Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.
Species of Protein	Human
Manufacturer - Search Terms	TNF-a, Tumor necrosis factor alpha, TNFA, TNFSF2, DIF (differentiation inducing factor ), CACHECTIN, DIF, TNF-alpha, Tumor necrosis factor alpha, Tumor necrosis factor alpha, TNF, tumor necrosis factor (TNF superfamily, member 2), TNF superfamily member 2, TNF0501-10ug, TNF0501-20ug, TNF0501-50ug, TNF0501-100ug, TNF0501-250ug, TNF0501-500ug, TNF0501-1mg, TNF0501-10, TNF0501-20, TNF0501-50, TNF0501-100, TNF0501-250, TNF0501-500, TNF0501-1

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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NKM-TNF0501-100ug-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 100 ug

lieferbar

Lieferung vsl. bis 31.07.2025