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Recombinant human TNF-alpha protein

Recombinant human TNF-alpha protein

Item no.	NKM-TNF0501-100ug
Manufacturer	NKMAX
Amount	100 ug
Quantity options	100 ug 20 ug 250 ug 500 ug 50 ug
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis
Type	Proteins Recombinant
Format	Liquid
Applications	SDS-PAGE
Specific against	Human (Homo sapiens)
Host	E.coli
Conjugate/Tag	Untagged
Purity	> 95% by SDS-PAGE
Sequence	MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Citations	Chicoine M.R., et al. (1997), J. Neurooncol. 35: 249-57 Ruddle N.H., et al. (1992), Curr. Opin. Immunol. 4: 327-32 Wingfield P., et al. (1987), FEBS Lett. 211:179-184 Kim JA, et al. A new lupane-type triterpene from the seeds of Panax ginseng with its inhibition of NF-κB. (Arch Pharm Res. 2012){https://pubmed.ncbi.nlm.nih.gov/22553057/} Nhiem NX, et al. Inhibition of nuclear transcription factor-κB and activation of peroxisome proliferator-activated receptors in HepG2 cells by cucurbitane-type triterpene glycosides from Momordica charantia. (J Med Food. 2012){https://pubmed.ncbi.nlm.nih.gov/22248180/} Song SB, et al. Inhibition of TNF-α-mediated NF-κB Transcriptional Activity in HepG2 Cells by Dammarane-type Saponins from Panax ginseng Leaves. (J Ginseng Res. 2012){https://pubmed.ncbi.nlm.nih.gov/23717114/} Liang C, et al. Oleanane-triterpenoids from Panax stipuleanatus inhibit NF-κB. (J Ginseng Res. 2013){https://pubmed.ncbi.nlm.nih.gov/23717159/} Cho K, et al. Inhibition of TNF-α-Mediated NF-κB Transcriptional Activity by Dammarane-Type Ginsenosides from Steamed Flower Buds of Panax ginseng in HepG2 and SK-Hep1 Cells. (Biomol Ther (Seoul). 2014){https://pubmed.ncbi.nlm.nih.gov/24596622/} Thao NP, et al. Inhibition of NF-κB transcriptional activation in HepG2 cells by diterpenoids from the soft coral Sinularia maxima. (Arch Pharm Res. 2014){https://pubmed.ncbi.nlm.nih.gov/23934574/} Thao NP, et al. NF-κB inhibitory activity of polyoxygenated steroids from the Vietnamese soft coral Sarcophyton pauciplicatum. (Bioorg Med Chem Lett. 2014){https://pubmed.ncbi.nlm.nih.gov/24852121/} Li W, et al. Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana. (Biomol Ther (Seoul). 2014){https://pubmed.ncbi.nlm.nih.gov/25143813/} He F, et al. Antitumor effects of dammarane-type saponins from steamed Notoginseng. (Pharmacogn Mag. 2014){https://pubmed.ncbi.nlm.nih.gov/25210319/} Yang SY, et al. NF-κB Activation and PPAR Transactivational Effects of a New Aliphatic Acid Amide from Pericarps of Zanthoxylum piperitum. (Bull Korean Chem Soc. 2014){https://doi.org/10.5012/bkcs.2014.35.8.2361} Sun YN, et al. NF-κB Inhibitory Activities of Phenolic and Lignan Components from the Stems of Acanthopanax divaricatus var. albeofructus. (Natural Product Sciences. 2014){https://koreascience.kr/article/JAKO201402852974800.page} Sun YN, et al. Nuclear Factor Kappa B Activation and Peroxisome Proliferator-activated Receptor Transactivational Effects of Chemical Components of the Roots of Polygonum multiflorum. (Pharmacogn Mag. 2016){https://pubmed.ncbi.nlm.nih.gov/27019559/} Sun YN, et al. Isolation and identification of chromone and pyrone constituents from Aloe and their anti-inflammatory activities. (J Funct Foods. 2016){https://doi.org/10.1016/j.jff.2015.12.026} Sun YN, et al. A new phenolic derivative with soluble epoxide hydrolase and nuclear factor-kappaB inhibitory activity from the aqueous extract of Acacia catechu. (Nat Prod Res. 2016){https://pubmed.ncbi.nlm.nih.gov/26647286/} Kwak MS, et al. Peroxiredoxin-mediated disulfide bond formation is required for nucleocytoplasmic translocation and secretion of HMGB1 in response to inflammatory stimuli. (Redox Biol. 2019){https://pubmed.ncbi.nlm.nih.gov/31026770/} Jang SH, et al. Sinapic acid alleviates inflammatory bowel disease (IBD) through localization of tight junction proteins by direct binding to TAK1 and improves intestinal microbiota. (Front Pharmacol. 2023){https://pubmed.ncbi.nlm.nih.gov/37649894/} Kim S, et al. Hybrid nutraceutical of 2-ketoglutaric acid in improving inflammatory bowel disease: Role of prebiotics and TAK1 inhibitor. (Biomed Pharmacother. 2024){https://pubmed.ncbi.nlm.nih.gov/38219386/}
NCBI	NP_000585.2
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Tumor necrosis factor,Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2,TNFSF2,TNF-a,TNFA,TNF
Similar products	TNF-alpha, TNF, TNF-a, TNFA, TNFSF2, DIF, member 2), TNF superfamily member 2, Tumor necrosis factor alpha, DIF (differentiation inducing factor ), CACHECTIN, tumor necrosis factor (TNF superfamily, TNF0501-10ug, TNF0501-20ug, TNF0501-50ug, TNF0501-100ug, TNF0501-250ug, TNF0501-500ug, TNF0501-1mg, TNF0501-10, TNF0501-20, TNF0501-50, TNF0501-100, TNF0501-250, TNF0501-500, TNF0501-1
Available
Manufacturer - Type	Recombinant Protein
Manufacturer - Applications	SDS-PAGE, Bioactivity
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Molecular Weight	17.5 kDa (158aa) confirmed by MALDI-TOF
Description	Tumor necrosis factor alpha (TNF-alpha), also called cachectin, consists of 157 amino acids. TNF-alpha is a 17. 5 kD factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-alpha is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Recombinant human TNF-alpha was expressed in E. coli and purified by using conventional chromatography techniques. Additional amino acid, methionine, was attached at N-terminus of the protein.
Concentration	1mg/ml (determined by Bradford assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4)
Antigen Species	Human
Gene Category	Hormones & Cytokines
Domain	77-233aa
Endotoxin	< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity Comment Only	Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.
Species of Protein	Human
Manufacturer - Search Terms	TNF-a, Tumor necrosis factor alpha, TNFA, TNFSF2, DIF (differentiation inducing factor ), CACHECTIN, DIF, TNF-alpha, Tumor necrosis factor alpha, Tumor necrosis factor alpha, TNF, tumor necrosis factor (TNF superfamily, member 2), TNF superfamily member 2, TNF0501-10ug, TNF0501-20ug, TNF0501-50ug, TNF0501-100ug, TNF0501-250ug, TNF0501-500ug, TNF0501-1mg, TNF0501-10, TNF0501-20, TNF0501-50, TNF0501-100, TNF0501-250, TNF0501-500, TNF0501-1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 9/4/2025

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