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CheKine™ Micro Mitochondrial complex Ⅲ Activity Assay Kit

ArtNr ABK-KTB1870-96T
Hersteller Abbkine Scientific
Menge 96T
Kategorie
Typ Assay
Specific against other
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Lieferbar
Applications Notes
Abbkine CheKine™ Micro Mitochondrial Complex Ⅲ Activity Detection Kitis specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. Complex III could transfer the hydrogen from reduced CoQ to cytochrome C to produce reduced cytochrome C. Unlike oxidized cytochrome C, reduced cytochrome C has characteristic light absorption at 550 nm. So, the increasing of light absorption rate can reflect the activity of Complex III enzymes, and Abbkine CheKine™ Micro Mitochondrial complex Ⅲ Activity Assay Kit can detect animal and plant tissues and cell samples.
Kit components
• ReagentⅠ
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Reagent Ⅴ
• Reagent Ⅵ
Features & Benefits
• Detect the enzyme activity of complex III in plant tissues, animal tissues, and cell samples.
• Provides detailed sample preparation and results calculation methods.
Usage Notes
• Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
• Avoid bubbles while mixing or redissolving components.
• Change pipette tips frequently to avoid cross contamination between components.
• Ensure that all components and equipment are at the proper temperature before starting the experiment.
• In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples. If the absorbance values is too high (above 1), the samples should be diluted with ReagentⅡand then measured again . Pay attention to multiply by the dilution factor when calculating the result.
• The protein concentration of the sample needs to be determined by users.
•Note that Reagent Ⅲ has certain toxicity, Please take protective measures during operation.
Storage Instructions
The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Mitochondrial respiratory chain complex III (EC 1.10.2.2), also known as CoQ-cytochrome C reductase, is widely present in the mitochondria of animals, plants, microorganisms and cultured cells. It plays a key role at the main and branch pathways in the mitochondrial respiratory electron transport chain. Complex III could transfer the hydrogen of the reduced CoQ to cytochrome C , and produce reduced cytochrome C.
Booklet
KTB1870-B.pdf
Tag
complex Ⅲ
Description
Abbkine CheKine™ Micro Mitochondrial Complex Ⅲ Activity Detection Kit is specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate.

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96T
Lieferbar: In stock
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