CheKine™ Micro Mitochondrial complex III Activity Assay Kit

Cell metabolism detection kit

Cell analysis

Gel pack with blue ice.

Mitochondrial respiratory chain complex III (EC 1.10.2.2), also known as CoQ-cytochrome C reductase, is widely present in the mitochondria of animals, plants, microorganisms and cultured cells. It plays a key role at the main and branch pathways in the mitochondrial respiratory electron transport chain. Complex III could transfer the hydrogen of the reduced CoQ to cytochrome C, and produce reduced cytochrome C.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
• Avoid bubbles while mixing or redissolving components.
• Change pipette tips frequently to avoid cross contamination between components.
• Ensure that all components and equipment are at the proper temperature before starting the experiment.
• In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples. If the absorbance values is too high (above 1), the samples should be diluted with ReagentIIand then measured again . Pay attention to multiply by the dilution factor when calculating the result.
• The protein concentration of the sample needs to be determined by users.
• Note that Reagent III has certain toxicity, Please take protective measures during operation.