GlpE, Recombinant, E.coli, aa1-108, His-Tag (Thiosulfate Cyanide Sulfurtransferase (Rhodanese), ECK3411, JW3388)

Blue Ice

14, 5

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol.

Thiosulfate:cyanide sulfurtransferase (rhodanese), also known as glpE, is a mitochondrial matrix enzyme that is encoded by the nucleus. glpE catalyzes the sulfur-transfer reaction in which a sulfur atom is transferred from thiosulfate to cyanide by a double-displacement mechanism. Escherichia coli glpE is a prototype for the single-domain rhodanese superfamily.

Source:
Recombinant protein corresponding to E.coli glpE, fused to His-tag at N-terminal expressed in E.coli.

Molecular Weight:
~14.5kD (131aa) (MALDI-TOF)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMDQFECI NVADAHQKLQ EKEAVLVDIR DPQSFAMGHA VQAFHLTNDT LGAFMRDNDF DTPVMVMCYH GNSSKGAAQY LLQQGYDVVY SIDGGFEAWQ RQFPAEVAYG A

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.