POLR2E, Recombinant, Human, aa1-210, His-Tag (DNA-directed RNA Polymerases I, II, and III Subunit RPABC1, hRPB25, hsRPB5, RPABC1, RPB5, XAP4)

Blue Ice

27, 1

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.1M sodium chloride, 1mM DTT, 10% glycerol.

POLR2E, also as known as DNA-directed RNA polymerases I, II, and III subunit RPABC1, belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. POLR2E is the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. POLR2E is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Source:
Recombinant protein corresponding to aa1-210 from human POLR2E at N-terminal, fused to His-tag expressed in E.coli.

Molecular Weight:
~27.1kD (233aa)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMDDEEET YRLWKIRKTI MQLCHDRGYL VTQDELDQTL EEFKAQFGDK PSEGRPRRTD LTVLVAHNDD PTDQMFVFFP EEPKVGIKTI KVYCQRMQEE NITRALIVVQ QGMTPSAKQS LVDMAPKYIL EQFLQQELLI NITEHELVPE HVVMTKEEVT ELLARYKLRE NQLPRIQAGD PVARYFGIKR GQVVKIIRPS ETAGRYITYR LVQ

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.