Pepsinogen II (PGII, PGC, Pepsinogen C, EC 3.4.23.3, Gastricin), Human

Blue Ice

40

Supplied as a lyophilized powder in Tris-chloride, pH 8.5

Pepsinogen II or pepsinogen C belongs to a group of aspartic proteinase zymogens (inactive form) that are activated in the acid pH of stomach to active enzyme pepsin. Pepsinogen II (PG II) is produced by chief cells and mucus neck cells of gastric mucosa, pyloric glands in the antrum and Brunner's glands in the proximal duodenum. Serum concentration of PG II reflects the histological status of gastric mucosa. The ratio of concentration of PGI to PGII in serum of normal subjects is about 4:1. The PGI/PGII ratio decreases linearly with increasing grade of atrophic gastritis in the corpus. The ratio is < 2.5 when the atrophic gastritis is advanced (moderate or severe) in the gastric corpus.

Solubility:
Clear colorless liquid at 1mg/mL in saline

Specific Activity:
Lot-dependent. ≥1, 000U/mg protein (upon activation)
Note: Pepsinogen self-activates upon exposure to low pH.

Unit Definition of Activity:
One unit will produce a ΔA280 of 0.001 per minute at pH 2.0 and 37°C, measured as TCA-soluble products from hemoglobin.

Storage and Stability:
Store lyophilized powder at -20°C for 12 months after receipt. After reconstitution maintain at or above pH 8.0 to avoid activation. For maximum recovery of product, centrifuge the original vial prior to removing the cap.