RND3, Recombinant, Human, aa1-241, His-Tag (Rho-related GTP-binding Protein RhoE, Protein MemB, Rho Family GTPase 3, Rho-related GTP-binding Protein Rho8, Rnd3, ARHE, RHO8, RHOE)

Blue Ice

29, 2

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 40% glycerol, 1mM EDTA, 0.1M sodium chloride.

Rho-related GTP-binding protein RhoE, also known as RND3, is a small GTPase of the Ras superfamily that regulate the organization of the actin cytoskeleton in response to extracellular growth factors. RND3 activity leads to a decrease in stress fibers and increased cell migration, at least in part through regulation of the Rho-dependent kinase ROCK1. Also, RND3 has been implicated in inhibition of DNA-damage induced apoptosis and cell cycle arrest.

Source:
Recombinant corresponding to aa1-241 of human RND3 protein, fused to His-tag at N-terminus, expressed in E.coli.

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MKERRASQKL SSKSIMDPNQ NVKCKIVVVG DSQCGKTALL HVFAKDCFPE NYVPTVFENY TASFEIDTQR IELSLWDTSG SPYYDNVRPL SYPDSDAVLI CFDISRPETL DSVLKKWKGE IQEFCPNTKM LLVGCKSDLR TDVSTLVELS NHRQTPVSYD QGANMAKQIG AATYIECSAL QSENSVRDIF HVATLACVNK TNKNVKRNKS QRATKRISHM PSRPELSAVA TDLRKDKAKS C

Molecular Weight:
29.2kD (261aa) confirmed by MALDI-TOF

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.