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Biotinylated Rat CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)

Biotinylated Rat CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)

ArtNr	CDG-R82D4-25ug
Hersteller	ACROBiosystems
Menge	25 ug
Quantity options	200 ug 25 ug
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins Recombinant
Format	Solid
Specific against	Rat (Rattus norvegicus)
Host	HEK293
Konjugat/Tag	Biotin, FLAG, HIS, Avi
Purity	90%
NCBI	NP_001101610.1 (CD3E) , NP_001071114.1 (CD3G)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Category	Protein / MABSol® Biotin Labeled Proteins
Manufacturer - Conjugate / Tag	C-10xHis & C-Avi \| C-Flag, Biotin-labeled
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	16.7 kDa & 15.7 kDa
Manufacturer - Format	Powder
Description	Biotinylated Rat CD3E&CD3G Heterodimer Protein, His, Avitag&Flag Tag (CDG-R82D4) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Asp 103 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_001101610.1 (CD3E) & NP_001071114.1 (CD3G)).
Background	T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Molecule	CD3E & CD3G
Exp Region	Gln 21 - Asp 103 (CD3E) & Gln 23 - Ser 116 (CD3G)
Characteristics	Biotinylated Rat CD3E&CD3G Heterodimer Protein, His, Avitag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.7 kDa (CD3E) and 15.7 kDa (CD3G). Subunit CD3E carries a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 22-25 kDa under reducing (R) condition, and 40-45 kDa under non-reducing (NR) condition (SDS-PAGE).
Endotoxin	1.0 EU per μg
Buffer	PBS, pH 7.4
Stability	● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

CDG-R82D4-25ug-datasheet.pdf

CDG-R82D4-25ug-safety-datasheet.pdf