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Biotinylated Rat CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)

Biotinylated Rat CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)

Item no.	CDG-R82D4-25ug
Manufacturer	ACROBiosystems
Amount	25 ug
Quantity options	200 ug 25 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Solid
Specific against	Rat (Rattus norvegicus)
Host	HEK293
Conjugate/Tag	Biotin, FLAG, HIS, Avi
Purity	90%
NCBI	NP_001101610.1 (CD3E) , NP_001071114.1 (CD3G)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Shipping Condition	Room temperature
Available
Manufacturer - Category	Protein / MABSol® Biotin Labeled Proteins
Manufacturer - Conjugate / Tag	C-10xHis & C-Avi \| C-Flag, Biotin-labeled
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	16.7 kDa & 15.7 kDa
Manufacturer - Format	Powder
Description	Biotinylated Rat CD3E&CD3G Heterodimer Protein, His, Avitag&Flag Tag (CDG-R82D4) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Asp 103 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_001101610.1 (CD3E) & NP_001071114.1 (CD3G)).
Background	T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Molecule	CD3E & CD3G
Exp Region	Gln 21 - Asp 103 (CD3E) & Gln 23 - Ser 116 (CD3G)
Characteristics	Biotinylated Rat CD3E&CD3G Heterodimer Protein, His, Avitag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.7 kDa (CD3E) and 15.7 kDa (CD3G). Subunit CD3E carries a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 22-25 kDa under reducing (R) condition, and 40-45 kDa under non-reducing (NR) condition (SDS-PAGE).
Endotoxin	1.0 EU per μg
Buffer	PBS, pH 7.4
Stability	● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

CDG-R82D4-25ug-datasheet.pdf

CDG-R82D4-25ug-safety-datasheet.pdf