CD272, Recombinant, Human, aa31-157 (BTLA, B- and T-lymphocyte attenuator)

Molecular Biology / MB-CD Markers

-20°C

Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to 100ug/ml.

BTLA is a type I transmembrane glycoprotein of the CD28 family of T cell costimulatory molecules. BTLA functions as a coinhibitor on activated T cells and on B cells and dendritic cells. The extracellular domain (ECD) of this Ig superfamily protein contains one V-type Ig-like domain, which interacts with HVEM.

Source:
Recombinant protein corresponding to aa31-157 from human CD272, fused to the N-terminus of the Fc region of mouse IgG2a, expressed in CHO cells.

Biological Activity:
Measured in a competitive binding assay.

Endotoxin:
~0.06EU/ug protein (LAL test; Lonza)

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.