Human Atherosclerosis Array C1 (4)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-ATH-1-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Atherosclerosis Antibody Array 1 Kit. Detects 20 Human Atherosclerotic Factors. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
20
Target Names
Eotaxin-1 (CCL11), GCSF, GDNF, GM-CSF, ICAM-1 (CD54), IFN-gamma, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, MCP-1 (CCL2), M-CSF, TGF beta 1, TGF beta 2, TGF beta 3, TNF alpha, TNF beta (TNFSF1B), MIP-3 alpha (CCL20), PDGF-BB, RANTES (CCL5)
Targets Detected
CCL11, CCL2, CCL20, CD54, Eotaxin-1, G-CSF, GDNF, GM-CSF, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 R1, IL-1 R2, M-CSF, MCP-1, MIP-3 alpha, PDGF-BB, RANTES, TGF beta 1, TGF beta 2, TGF beta 3, TNF alpha, TNF beta, TNFSF1B
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Atherosclerosis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Woroniecki R P, Shatat I F, Supe K, Du Z F, Frederick J K.Urinary Cytokines and Steroid Responsiveness in Idiopathic Nephrotic Syndrome of Childhood. Am J Nephrol. 2008, 28:83–90
Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 542,97 €
Preis: 542,97 €
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