Human Cytokine Array G1000 (8)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-CYT-G1000-8
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio G-Series Human Cytokine Antibody Array 1000 Kit. A combination of Human Cytokine Antibody Array G6 & G7. Detects 120 Human Cytokines. Suitable for all liquid sample types.
Features
  • High sensitivity and specificity
  • Low sample volume (10-100 ul per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Number of Targets Detected
120
Target Names
Adiponectin (ACRP30), AgRP, Amphiregulin, Angiogenin, Angiopoietin-2, Axl, BDNF, bFGF, BLC (CXCL13), BMP-4, BMP-6, beta-NGF, Betacellulin (BTC), CCL28 (MEC), CK beta 8-1 (CCL23), CNTF, CTACK (CCL27), Dtk, EGF, EGFR, ENA-78 (CXCL5), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), Fas (TNFRSF6/Apo-1), FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, Fractalkine (CX3CL1), GCP-2 (CXCL6), GCSF, GDNF, GITR (TNFRSF18), GITR Ligand (TNFSF18), GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), HCC-4 (CCL16), HGF, I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-3 (CD50), IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-1 R, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17A, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 ra (IL-1 F3), IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), I-TAC (CXCL11), Leptin, Light (TNFSF14), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, MDC (CCL22), MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NT-3, NT-4, Oncostatin M, Osteoprotegerin (TNFRSF11B), PARC (CCL18), PDGF-BB, PLGF, RANTES (CCL5), SCF, SDF-1 alpha (CXCL12 alpha), gp130, TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TARC (CCL17), TECK (CCL25), TGF beta 3, TGF beta 1, Thrombopoietin (TPO), TIMP-1, TIMP-2, TNF alpha, TNF beta (TNFSF1B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), uPAR, VEGF-A, VEGF-D
Targets Detected
ACRP30, Adiponectin, AgRP, Amphiregulin, Angiogenin, Angiopoietin-2, Apo-1, AXL, BDNF, beta-NGF, Betacellulin, bFGF, BLC, BMP-4, BMP-6, BTC, CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CD50, CD54, CK beta 8-1, CNTF, CTACK, CX3CL1, CXCL1, CXCL11, CXCL12 alpha, CXCL13, CXCL5, CXCL6, CXCL7, CXCL8, CXCL9, Dtk, EGF, EGFR, ENA-78, Eotaxin-1, Eotaxin-2, Eotaxin-3, ErbB1, Fas, FGF-4, FGF-6, FGF-7, FGF-9, Flt-3 Ligand, Fractalkine, G-CSF, GCP-2, GDNF, GITR, GITR Ligand, GM-CSF, gp130, GRO, GRO alpha, HCC-4, HGF, I-309, I-TAC, ICAM-1, ICAM-3, IFN-gamma, IGF-1, IGF-1 R, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 F3, IL-1 R1, IL-1 R4, IL-1 Ra, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17, IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-8, KGF, Leptin, LIGHT, Lymphotactin, M-CSF, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MEC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MIP-3 beta, MPIF-2, MSP alpha/beta, NAP-2, NT-3, NT-4, Oncostatin M, Osteoprotegerin, PARC, PDGF-BB, PlGF, PPBP, RANTES, SCF, SDF-1 alpha, ST2, TARC, TCA-3, TECK, TGF beta 1, TGF beta 3, Thrombopoietin, TIMP-1, TIMP-2, TNF alpha, TNF beta, TNF RI, TNF RII, TNFRSF10D, TNFRSF11B, TNFRSF18, TNFRSF6, TNFSF14, TNFSF18, TNFSF1B, TPO, TRAIL R3, TRAIL R4, uPAR, VEGF-A, VEGF-D, XCL1
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components
  • Human Cytokine Antibody Array G1000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Mahadevan D., et al. Gene Expression and Serum Cytokine Profiling of Low Stage CLL Identify WNT/PCP, Flt-3L/Flt-3, and CXCL9/CXCR3 as Regulators of Cell Proliferation, Survival, and Migration. Hum Genomics Proteomics. 2009 Jun 24, 2009:453634. doi: 10.4061/2009/453634.
2. Lis R., et al. Tumor associated mesenchymal stem cells protects ovarian cancer cells from hyperthermia through CXCL12. Int. J. Cancer: 128, 715–725 (2011). DOI: 10.1002/ijc.25619
3. De Ceuninck F, Marcheteau E, Berger S, Caliez A, et al. Assessment of Some Tools for the Characterization of the Human Osteoarthritic Cartilage Proteome. J Biomol Tech. 2005, 16:256–265.
Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 2.319,01 €
Preis: 2.319,01 €
lieferbar

Vergleichen

Angebot anfordern

Fragen zum Produkt?
 
Schließen