Hersteller |
Raybiotech
|
Kategorie |
|
Typ |
Assay |
Specific against |
Human |
Menge |
2 Sample Kit |
ArtNr |
AAH-PER-1-2 |
Targets |
ERBB2;;ERBB3 |
eClass 6.1 |
32161000 |
eClass 9.0 |
32161000 |
Lieferbar |
|
Short description |
RayBio C-Series Human EGFR Phosphorylation Antibody Array 1 Kit. Detects 17 site specific and pan Human EGFRs. Suitable for all liquid sample types but intended for use with cell and tissue lysates. |
Secondary categories |
Products, Functional Assays, Phosphorylation Assays, Phosphorylation Arrays |
Features |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
|
Number of Targets Detected |
17 |
Target Names |
EGFR (P-Tyr845), EGFR (P-Tyr992), EGFR (P-Tyr1045), EGFR (P-Tyr1068), EGFR (P-Tyr1086), EGFR (P-Tyr1148), EGFR (P-Tyr1173), EGFR (P-Ser1046/1047), EGFR (P-Ser1070), ErbB2 (P-Tyr877), ErbB2 (P-Tyr1112), ErbB2 (P-Tyr 1221/1222), ErbB2 (P-Tyr1248), ErbB2 (P-Thr686), ErbB2 (P-Ser1113), ErbB3 (P-Tyr1289), ErbB4 (P-Tyr1284) |
Targets Detected |
EGFR (P-Ser1046/1047), EGFR (P-Ser1070), EGFR (P-Tyr1045), EGFR (P-Tyr1068), EGFR (P-Tyr1086), EGFR (P-Tyr1148), EGFR (P-Tyr1173), EGFR (P-Tyr845), EGFR (P-Tyr992), ErbB1 (P-Ser1046/1047), ErbB1 (P-Ser1070), ErbB1 (P-Tyr1045), ErbB1 (P-Tyr1068), ErbB1 (P-Tyr1086), ErbB1 (P-Tyr1148), ErbB1 (P-Tyr1173), ErbB1 (P-Tyr845), ErbB1 (P-Tyr992), ErbB2 (P-Ser1113), ErbB2 (P-Thr686), ErbB2 (P-Tyr 1221/1222), ErbB2 (P-Tyr1112), ErbB2 (P-Tyr1248), ErbB2 (P-Tyr877), ErbB3 (P-Tyr1289), ErbB4 (P-Tyr1284) |
Suggested Applications |
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process |
Kit Components |
- Human EGFR Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail Set I
- Phosphatase Inhibitor Cocktail II
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
|
Other Materials Required |
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
|
Protocol Outline |
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
|
Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months. |
Solid Support |
Membrane |
Design Principle |
Sandwich-based |
Result Output |
Semi-Quantitative |
Detection Method |
Chemiluminescence |
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Citation |
1. Shalaby A., et al. The role of epidermal growth factor receptor in chordoma pathogenesis: a potential therapeutic target. J Pathol. 2011 Feb, 223(3):336-46. doi: 10.1002/path.2818. 2. Bhuvaneswari R., et al. Targeting EGFR with photodynamic therapy in combination with Erbitux enhances in vivo bladder tumor response., Mol Cancer. 2009 Nov 2, 8:94. doi: 10.1186/1476-4598-8-94. 3. Zhu S., et al. DARPP-32 Increases Interactions Between Epidermal Growth Factor Receptor and ERBB3 to Promote Tumor Resistance to Gefitinib. Gastroenterology. 2011 Nov, 141(5):1738-48.e1-2. doi: 10.1053/j.gastro.2011.06.070. 4. Cheng KW, Wong CC, Alston N, et al. Aerosol administration of phospho-sulindac inhibits lung tumorigenesis. Mol. Cancer Ther. 2013 May 3, 12: 1417-1428 |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.