Human EGFR Phosphorylation Array C1 (8)

Hersteller Raybiotech
Kategorie
Typ Assay
Specific against Human
Menge 8 Sample Kit
ArtNr AAH-PER-1-8
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human EGFR Phosphorylation Antibody Array 1 Kit. Detects 17 site specific and pan Human EGFRs. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Secondary categories
Products, Functional Assays, Phosphorylation Assays, Phosphorylation Arrays
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
17
Target Names
EGFR (P-Tyr845), EGFR (P-Tyr992), EGFR (P-Tyr1045), EGFR (P-Tyr1068), EGFR (P-Tyr1086), EGFR (P-Tyr1148), EGFR (P-Tyr1173), EGFR (P-Ser1046/1047), EGFR (P-Ser1070), ErbB2 (P-Tyr877), ErbB2 (P-Tyr1112), ErbB2 (P-Tyr 1221/1222), ErbB2 (P-Tyr1248), ErbB2 (P-Thr686), ErbB2 (P-Ser1113), ErbB3 (P-Tyr1289), ErbB4 (P-Tyr1284)
Targets Detected
EGFR (P-Ser1046/1047), EGFR (P-Ser1070), EGFR (P-Tyr1045), EGFR (P-Tyr1068), EGFR (P-Tyr1086), EGFR (P-Tyr1148), EGFR (P-Tyr1173), EGFR (P-Tyr845), EGFR (P-Tyr992), ErbB1 (P-Ser1046/1047), ErbB1 (P-Ser1070), ErbB1 (P-Tyr1045), ErbB1 (P-Tyr1068), ErbB1 (P-Tyr1086), ErbB1 (P-Tyr1148), ErbB1 (P-Tyr1173), ErbB1 (P-Tyr845), ErbB1 (P-Tyr992), ErbB2 (P-Ser1113), ErbB2 (P-Thr686), ErbB2 (P-Tyr 1221/1222), ErbB2 (P-Tyr1112), ErbB2 (P-Tyr1248), ErbB2 (P-Tyr877), ErbB3 (P-Tyr1289), ErbB4 (P-Tyr1284)
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human EGFR Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • Phosphatase Inhibitor Cocktail Set I
  • Phosphatase Inhibitor Cocktail II
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Shalaby A., et al. The role of epidermal growth factor receptor in chordoma pathogenesis: a potential therapeutic target. J Pathol. 2011 Feb, 223(3):336-46. doi: 10.1002/path.2818.
2. Bhuvaneswari R., et al. Targeting EGFR with photodynamic therapy in combination with Erbitux enhances in vivo bladder tumor response., Mol Cancer. 2009 Nov 2, 8:94. doi: 10.1186/1476-4598-8-94.
3. Zhu S., et al. DARPP-32 Increases Interactions Between Epidermal Growth Factor Receptor and ERBB3 to Promote Tumor Resistance to Gefitinib. Gastroenterology. 2011 Nov, 141(5):1738-48.e1-2. doi: 10.1053/j.gastro.2011.06.070.
4. Cheng KW, Wong CC, Alston N, et al. Aerosol administration of phospho-sulindac inhibits lung tumorigenesis. Mol. Cancer Ther. 2013 May 3, 12: 1417-1428
Menge: 8 Sample Kit
Lieferbar: In stock
Listenpreis: 721,60 €
Preis: 721,60 €
lieferbar

Vergleichen

Angebot anfordern

Fragen zum Produkt?
 
Schließen