Mouse Cytokine Array C4 (4)

Hersteller Raybiotech
Kategorie
Typ Assay
Specific against Mouse
Menge 4 Sample Kit
ArtNr AAM-CYT-4-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Mouse Cytokine Antibody Array 4 Kit. Detects 34 Mouse Cytokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
34
Target Names
bFGF, CD26 (DPPIV), Dtk, E-Selectin, Fc gamma RIIB (CD32b), Flt-3 Ligand, GITR (TNFRSF18), HGFR, ICAM-1 (CD54), IGFBP-2, IGF-1, IGF-2, IL-15, IL-17 RB, IL-7, I-TAC (CXCL11), Lungkine (CXCL15), MDC (CCL22), MMP-2, MMP-3, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), Pro-MMP-9, Resistin, Sonic Hedgehog N-Terminal (Shh-N), TCK-1 (CXCL7), TIMP-2, TRANCE (TNFSF11), TROY (TNFRSF19), TSLP, VEGFR1, VEGFR2, VEGFR3, VEGF-D
Targets Detected
bFGF, CCL22, CD26, CD32b, CD54, CXCL11, CXCL15, CXCL7, DPPIV, Dtk, E-Selectin, Fc gamma RIIB, Flt-3 Ligand, GITR, HGFR, I-TAC, ICAM-1, IGF-1, IGF-2, IGFBP-2, IL-15, IL-17B R, IL-7, Lungkine, MDC, MMP-2, MMP-3, Osteopontin, Osteoprotegerin, Pro-MMP-9, Resistin, Shh-N, Sonic Hedgehog N-Terminal, SPP1, TCK-1, TIMP-2, TNFRSF11B, TNFRSF18, TNFRSF19, TNFSF11, TRANCE, TROY, TSLP, VEGF-D, VEGFR1, VEGFR2, VEGFR3
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C4 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Cyclophilin D Extramitochondrial Signaling Controls Cell Cycle Progression and Chemokine-Directed Cell Motility
2. Sugano G., Bernard-Pierrot I., Lae M., Battail C., Allory Y., Stransky N., et al. Milk fat globule—epidermal growth factor—factor VIII (MFGE8)/lactadherin promotes bladder tumor development. Oncogene (2011) 30, 642–653,
3. Palmi C., et al. Cytoskeletal Regulatory Gene Expression and Migratory Properties of B Cell Progenitors are Affected by the ETV6-RUNX1 Rearrangement Mol Cancer Res molcanres.0056.2014, Published OnlineFirst July 24, 2014, doi: 10.1158/1541-7786.MCR-14-0056-T
Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 631,98 €
Preis: 631,98 €
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