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Mouse Cytokine Array C4

Mouse Cytokine Array C4

Hersteller	Raybiotech
Kategorie	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Molecular Targets for Neuroscience Aging & Neurological Disorders Neural Signaling Neural Development By Cell Type Blastoma Carcinoma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Adenocarcinoma Arrays By Organ Lung Cancer Bladder Cancer Testicular & Prostate Cancer Lymphatic System Cancer Neuronal Cell Markers Antibody Arrays
Typ	Assay
Specific against	Mouse
Menge	8 Sample Kit
ArtNr	AAM-CYT-4-8
Targets	KDR ;; SELE ;;CD32;;DPP4;;FCGR2B;;FLT1;;FLT3;;ICAM1;;IGFBP2;;IL15;;IL17A;;KRT1;;MMP2;;MMP3;;MMP9;;RETN;;TNFRSF11B;;TNFSF11;;VEGFA;;VEGFD
eClass 6.1	32161000
eClass 9.0	32161000
Lieferbar
Short description	RayBio C-Series Mouse Cytokine Antibody Array 4 Kit. Detects 34 Mouse Cytokines. Suitable for all liquid sample types.
Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected	34
Target Names	bFGF, CD26 (DPPIV), Dtk, E-Selectin, Fc gamma RIIB (CD32b), Flt-3 Ligand, GITR (TNFRSF18), HGFR, ICAM-1 (CD54), IGFBP-2, IGF-1, IGF-2, IL-15, IL-17 RB, IL-7, I-TAC (CXCL11), Lungkine (CXCL15), MDC (CCL22), MMP-2, MMP-3, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), Pro-MMP-9, Resistin, Sonic Hedgehog N-Terminal (Shh-N), TCK-1 (CXCL7), TIMP-2, TRANCE (TNFSF11), TROY (TNFRSF19), TSLP, VEGFR1, VEGFR2, VEGFR3, VEGF-D
Targets Detected	bFGF, CCL22, CD26, CD32b, CD54, CXCL11, CXCL15, CXCL7, DPPIV, Dtk, E-Selectin, Fc gamma RIIB, Flt-3 Ligand, GITR, HGFR, I-TAC, ICAM-1, IGF-1, IGF-2, IGFBP-2, IL-15, IL-17B R, IL-7, Lungkine, MDC, MMP-2, MMP-3, Osteopontin, Osteoprotegerin, Pro-MMP-9, Resistin, Shh-N, Sonic Hedgehog N-Terminal, SPP1, TCK-1, TIMP-2, TNFRSF11B, TNFRSF18, TNFRSF19, TNFSF11, TRANCE, TROY, TSLP, VEGF-D, VEGFR1, VEGFR2, VEGFR3
Suggested Applications	Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components	Mouse Cytokine Antibody Array C4 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated HRP Detection Buffer C Detection Buffer D Lysis Buffer 8-Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support	Membrane
Design Principle	Sandwich-based
Result Output	Semi-Quantitative
Detection Method	Chemiluminescence
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation	1. Cyclophilin D Extramitochondrial Signaling Controls Cell Cycle Progression and Chemokine-Directed Cell Motility 2. Sugano G., Bernard-Pierrot I., Lae M., Battail C., Allory Y., Stransky N., et al. Milk fat globule—epidermal growth factor—factor VIII (MFGE8)/lactadherin promotes bladder tumor development. Oncogene (2011) 30, 642–653, 3. Palmi C., et al. Cytoskeletal Regulatory Gene Expression and Migratory Properties of B Cell Progenitors are Affected by the ETV6-RUNX1 Rearrangement Mol Cancer Res molcanres.0056.2014, Published OnlineFirst July 24, 2014, doi: 10.1158/1541-7786.MCR-14-0056-T

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