|
Hersteller |
Raybiotech
|
|
Kategorie |
|
|
Typ |
Assay |
|
Specific against |
Rat |
|
Menge |
4 Sample Kit |
|
ArtNr |
AAR-CYT-G1-4 |
|
eClass 6.1 |
32161000 |
|
eClass 9.0 |
32161000 |
|
Lieferbar |
 |
| Short description |
RayBio G-Series Rat Cytokine Antibody Array 1 Kit. Detects 19 Rat Cytokines. Suitable for all liquid sample types. |
| Features |
- High sensitivity and specificity
- Low sample volume (10-100 ul per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
|
| Number of Targets Detected |
19 |
| Target Names |
CINC-2, CINC-3, CNTF, Fractalkine (CX3CL1), GM-CSF, IFN-gamma, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-4, IL-6, IL-10, LIX, Leptin, MCP-1 (CCL2), MIP-3 alpha (CCL20), beta-NGF, TIMP-1, TNF alpha, VEGF-A |
| Targets Detected |
beta-NGF, CCL2, CCL20, CINC-2, CINC-3, CNTF, CX3CL1, Fractalkine, GM-CSF, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-4, IL-6, Leptin, LIX, MCP-1, MIP-3 alpha, TIMP-1, TNF alpha, VEGF-A |
| Suggested Applications |
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity |
| Kit Components |
- Rat Cytokine Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
|
| Other Materials Required |
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
|
| Protocol Outline |
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
|
| Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months. |
| Solid Support |
Glass Slide |
| Design Principle |
Sandwich-based |
| Result Output |
Semi-Quantitative |
| Detection Method |
Fluorescence Laser Scanner |
| Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Citation |
Cohen TS., et al. Therapeutic potential of plasma gelsolin administration in a rat model of sepsis. Cytokine. 2011 Jun, 54(3):235-8. doi: 10.1016/j.cyto.2011.02.006. |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt und Sicherheitsdatenblatt) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
