|
Hersteller |
Raybiotech
|
|
Kategorie |
|
|
Typ |
Assay |
|
Specific against |
Rat |
|
Menge |
4 Sample Kit |
|
ArtNr |
AAR-CYT-G1-4 |
|
eClass 6.1 |
32161000 |
|
eClass 9.0 |
32161000 |
|
Lieferbar |
 |
| Short description |
RayBio G-Series Rat Cytokine Antibody Array 1 Kit. Detects 19 Rat Cytokines. Suitable for all liquid sample types. |
| Features |
- High sensitivity and specificity
- Low sample volume (10-100 ul per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
|
| Number of Targets Detected |
19 |
| Target Names |
CINC-2, CINC-3, CNTF, Fractalkine (CX3CL1), GM-CSF, IFN-gamma, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-4, IL-6, IL-10, LIX, Leptin, MCP-1 (CCL2), MIP-3 alpha (CCL20), beta-NGF, TIMP-1, TNF alpha, VEGF-A |
| Targets Detected |
beta-NGF, CCL2, CCL20, CINC-2, CINC-3, CNTF, CX3CL1, Fractalkine, GM-CSF, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-4, IL-6, Leptin, LIX, MCP-1, MIP-3 alpha, TIMP-1, TNF alpha, VEGF-A |
| Suggested Applications |
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity |
| Kit Components |
- Rat Cytokine Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
|
| Other Materials Required |
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
|
| Protocol Outline |
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
|
| Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months. |
| Solid Support |
Glass Slide |
| Design Principle |
Sandwich-based |
| Result Output |
Semi-Quantitative |
| Detection Method |
Fluorescence Laser Scanner |
| Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Citation |
Cohen TS., et al. Therapeutic potential of plasma gelsolin administration in a rat model of sepsis. Cytokine. 2011 Jun, 54(3):235-8. doi: 10.1016/j.cyto.2011.02.006. |
