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Rat Cytokine Array G1

Rat Cytokine Array G1

Hersteller	Raybiotech
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Neural Signaling By Cell Type Carcinoma Hematology Leukemia Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Adenocarcinoma Endocrine Regulation of Lipid Metabolism Arrays By Organ Bladder Cancer Antibody Arrays
Typ	Assay
Specific against	Rat
Menge	8 Sample Kit
ArtNr	AAR-CYT-G1-8
Targets	IL4 ;; IL6 ;; LEP ;;CCL2;;IL10;;IL1A;;IL1B;;TIMP1;;VEGFA
eClass 6.1	32161000
eClass 9.0	32161000
Lieferbar
Short description	RayBio G-Series Rat Cytokine Antibody Array 1 Kit. Detects 19 Rat Cytokines. Suitable for all liquid sample types.
Features	High sensitivity and specificity Low sample volume (10-100 ul per array) Large dynamic range of detection Compatible with most sample types Test 4 or 8 samples on each slide Suitable for high-throughput assays
Number of Targets Detected	19
Target Names	CINC-2, CINC-3, CNTF, Fractalkine (CX3CL1), GM-CSF, IFN-gamma, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-4, IL-6, IL-10, LIX, Leptin, MCP-1 (CCL2), MIP-3 alpha (CCL20), beta-NGF, TIMP-1, TNF alpha, VEGF-A
Targets Detected	beta-NGF, CCL2, CCL20, CINC-2, CINC-3, CNTF, CX3CL1, Fractalkine, GM-CSF, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-4, IL-6, Leptin, LIX, MCP-1, MIP-3 alpha, TIMP-1, TNF alpha, VEGF-A
Suggested Applications	Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components	Rat Cytokine Antibody Array G1 Slide(s) Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated Fluor Lysis Buffer Adhesive Plastic Strips 30 ml Centrifuge Tube Manual
Other Materials Required	Small plastic boxes or containers Pipettors, pipette tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline	Dry the glass slide Block array surface Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with Streptavidin-Conjugated Fluor Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support	Glass Slide
Design Principle	Sandwich-based
Result Output	Semi-Quantitative
Detection Method	Fluorescence Laser Scanner
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation	Cohen TS., et al. Therapeutic potential of plasma gelsolin administration in a rat model of sepsis. Cytokine. 2011 Jun, 54(3):235-8. doi: 10.1016/j.cyto.2011.02.006.

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