« Zurück
Startseite /
Mouse IL-1 beta ELISA Kit

Mouse IL-1 beta ELISA Kit

ArtNr	ELM-IL1b-2
Hersteller	Raybiotech
Menge	2 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Cholesterol & Lipid Metabolism Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer
Typ	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Sensitivity	5 pg/ml
Citations	1. Wang X-A, Zhang R, Jiang D, et al. Interferon regulatory factor 9 protects against hepatic insulin resistance and steatosis in male mice. Hepatology. 2013 Aug,58(2):603–616. 2. Xu X., Shao B., Wang R., Zhou S., Tang Z., Lu W., Xiong S. Role of Interleukin-17 in defense against pseudomonas aeruginosa infection in lungs. Int J Clin Exp Med 2014,7(4):809-816 3. Bishayi B., Bandyopadhyay D., Majhi A., Adhikary R. POSSIBLE ROLE OF TOLL LIKE RECEPTOR-2 (TLR-2) IN THE INTRACELLULAR SURVIVAL OF STAPHYLOCOCCUS AUREUS IN MURINE PERITONEAL MACROPHAGES: INVOLVEMENT OF CYTOKINES AND ANTI-OXIDANT ENZYMES. Scandinavian Journal of Immunology. 2014, May Epub Ahead of Print, doi:10.1111/sji.12195 4. Tian X., et al. In Vivo Immunotoxicity of SiO2(Y0.5Gd0.45Eu0.05)2O3 as Dual-Modality Nanoprobes. Int. J. Mol. Sci. 2014, 15, 13649-13662, doi:10.3390/ijms150813649 5. Tian X., et al. Toxicity evaluation of Gd2O3SiO2 nanoparticles prepared by laser ablation in liquid as MRI contrast agents in vivo. International Journal of Nanomedicine. 2014 Aug 21,9:4043-53. doi: 10.2147/IJN.S66164. 6. Zhang Y., et al. Interferon regulatory factor 9 is an essential mediator of heart dysfunction and cell death following myocardial ischemia/reperfusion injury. Basic Res Cardiol (2014) 109:434. DOI 10.1007/s00395-014-0434-9 7. Yu L., et al. MRTF-A mediates LPS-induced pro-inflammatory transcription by interacting with the COMPASS complex. Journal of Cell Science. Advance Online Article September 4, 2014 doi: 10.1242/jcs.152314 8. Yu L., et al. MRTF-A mediates LPS-induced pro-inflammatory transcription by interacting with the COMPASS complex. Journal of Cell Science. Advance Online Article September 4, 2014 doi: 10.1242/jcs.152314 9. Reza Mahmoudian Sani, M. et al. Investigation of caspase-1 activity and interleukin-1beta production in murine macrophage cell lines infected with Leishmania major. Asian Pacific Journal of Tropical Biomedicine 2014. Epub Ahead of Print DOI: 10.12980/APJTB.4.2014APJTB-2014-0052 10. Liu J., et al. Sub-10 nm Monoclinic Gd2O3:Eu3+ Nanoparticles as Dual-Modal Nanoprobes for Magnetic Resonance and Fluorescence Imaging. Langmuir, Article ASAP, 2014. DOI: 10.1021/la503228v 11. Li R.L., et al. Postoperative impairment of cognitive function in old mice: a possible role for neuroinflammation mediated by HMGB1, S100B, and RAGE. Journal of Surgical Research, 185(2013) 815 -824. http://dx.doi.org/10.1016/j.jss.2013.06.043
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	IL-1 beta, IL-1 F2
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	IL-1 beta / IL-1F2
Shipping Temperature	Blue ice
Storage Conditions	≤-20°C
Description	RayBio® Mouse IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	IL1B
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
UNSPSC Code	41116158
Result Output	Quantitative
Protein Name & Synonyms	Interleukin-1 beta (IL-1 beta)
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Recommended Dilution	2 fold

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

ELM-IL1b-2.pdf

ELM-IL1b-2-datasheet.pdf

ELM-IL1b-2-safety-datasheet.pdf